Method Based on the β -Lactamase PenPC Fluorescent Labeled for β -Lactam Antibiotic Quantification in Human Plasma

Max Andresen; Kwok Yin Wong; Yun Chung Leung; Wai Ting Wong; Pak Ho Chan; Max Andresen-Vasquez; Leyla Alegria; Camila Silva; Pablo Tapia; Patricio Downey; Dagoberto Soto

doi:10.1155/2016/4307987

Method Based on the β -Lactamase PenPC Fluorescent Labeled for β -Lactam Antibiotic Quantification in Human Plasma

Max Andresen, Kwok Yin Wong, Yun Chung Leung, Wai Ting Wong, Pak Ho Chan, Max Andresen-Vasquez, Leyla Alegria, Camila Silva, Pablo Tapia, Patricio Downey, Dagoberto Soto

Research output: Journal article publication › Journal article › Academic research › peer-review

7 Citations (Scopus)

Abstract

Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.

Original language	English
Article number	4307987
Journal	BioMed Research International
Volume	2016
DOIs	https://doi.org/10.1155/2016/4307987
Publication status	Published - 1 Jan 2016

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1155/2016/4307987

http://hdl.handle.net/10397/43398

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title = "Method Based on the β -Lactamase PenPC Fluorescent Labeled for β -Lactam Antibiotic Quantification in Human Plasma",

abstract = "Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.",

author = "Max Andresen and Wong, {Kwok Yin} and Leung, {Yun Chung} and Wong, {Wai Ting} and Chan, {Pak Ho} and Max Andresen-Vasquez and Leyla Alegria and Camila Silva and Pablo Tapia and Patricio Downey and Dagoberto Soto",

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T1 - Method Based on the β -Lactamase PenPC Fluorescent Labeled for β -Lactam Antibiotic Quantification in Human Plasma

AU - Andresen, Max

AU - Wong, Kwok Yin

AU - Leung, Yun Chung

AU - Wong, Wai Ting

AU - Chan, Pak Ho

AU - Andresen-Vasquez, Max

AU - Alegria, Leyla

AU - Silva, Camila

AU - Tapia, Pablo

AU - Downey, Patricio

AU - Soto, Dagoberto

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.

AB - Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.

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DO - 10.1155/2016/4307987

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JO - BioMed Research International

JF - BioMed Research International

M1 - 4307987

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Method Based on the β -Lactamase PenPC Fluorescent Labeled for β -Lactam Antibiotic Quantification in Human Plasma

Abstract

ASJC Scopus subject areas

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