TY - JOUR
T1 - Mediators of corneal haze following implantation of presbyopic corneal inlays
AU - Fenner, Beau J.
AU - Liu, Yu Chi
AU - Koh, Siew Kwan
AU - Gao, Yan
AU - Deng, Lu
AU - Beuerman, Roger W.
AU - Zhou, Lei
AU - Theng, Julian T.S.
AU - Mehta, Jodhbir S.
N1 - Funding Information:
Supported by Proteomics Core Facility at Singapore Eye Research Institute, Singapore National Medical Research Council’s (NMRC) Centre Grant CG 2017, and Revision Optics research grant to Singapore Eye Research Institute. Disclosure: B.J. Fenner, None; Y.-C. Liu, None; S.K. Koh, None; Y. Gao, None; L. Deng, None; R.W. Beuerman, None; L. Zhou, None; J.T.S. Theng, None; J.S. Mehta, None
Funding Information:
Supported by Proteomics Core Facility at Singapore Eye Research Institute, Singapore National Medical Research Council’s (NMRC) Centre Grant CG 2017, and Revision Optics research grant to Singapore Eye Research Institute.
Publisher Copyright:
© 2019 The Authors.
PY - 2019/3
Y1 - 2019/3
N2 - PURPOSE. To identify protein mediators of corneal haze following presbyopic corneal inlay surgery. METHODS. Tears were collected from eyes with corneal haze following surgery with a shape-changing corneal inlay. Samples were subjected to quantitative proteomic analysis using iTRAQ and proteins significantly increased or decreased (1.3-fold or more) in haze eyes relative to fellow eyes were identified. Expression ratios were compared to postoperative eyes without corneal haze to identify proteins selectively increased or decreased in corneal haze eyes. RESULTS. Inlay-associated haze occurred in 35% of eyes (6 of 17). Of 1443 unique tear proteins identified, eight proteins were selectively reduced in tears from postoperative haze eyes and one protein selectively increased. Proteins reduced in haze eyes included complement 4a (level relative to nonhaze eyes 0.464, P = 0.037), complement factor H (0.589, P = 0.048), immunoglobulin kappa variable 2–29 (0.128, P = 0.006), immunoglobulin kappa variable 2D-28 (0.612, P = 0.025), immunoglobulin lambda variable 7–46 (0.482, P = 0.007), S100 calcium binding protein A4 (0.614, P = 0.048), Shootin-1 (0.614, P = 0.048), and tissue inhibitor of metalloproteinase-1 (0.736, P = 0.023). The Xaa-Pro aminopeptidase 1 was increased in haze eyes relative to nonhaze eyes (1.517, P = 0.023). CONCLUSIONS. Corneal haze following corneal inlay surgery is associated with reduction in levels of known inflammatory and immune mediators. These findings represent a starting point for elucidation of pathways involved in corneal haze following synthetic inlay implantation and may enable development of targeted therapies that modulate the haze response.
AB - PURPOSE. To identify protein mediators of corneal haze following presbyopic corneal inlay surgery. METHODS. Tears were collected from eyes with corneal haze following surgery with a shape-changing corneal inlay. Samples were subjected to quantitative proteomic analysis using iTRAQ and proteins significantly increased or decreased (1.3-fold or more) in haze eyes relative to fellow eyes were identified. Expression ratios were compared to postoperative eyes without corneal haze to identify proteins selectively increased or decreased in corneal haze eyes. RESULTS. Inlay-associated haze occurred in 35% of eyes (6 of 17). Of 1443 unique tear proteins identified, eight proteins were selectively reduced in tears from postoperative haze eyes and one protein selectively increased. Proteins reduced in haze eyes included complement 4a (level relative to nonhaze eyes 0.464, P = 0.037), complement factor H (0.589, P = 0.048), immunoglobulin kappa variable 2–29 (0.128, P = 0.006), immunoglobulin kappa variable 2D-28 (0.612, P = 0.025), immunoglobulin lambda variable 7–46 (0.482, P = 0.007), S100 calcium binding protein A4 (0.614, P = 0.048), Shootin-1 (0.614, P = 0.048), and tissue inhibitor of metalloproteinase-1 (0.736, P = 0.023). The Xaa-Pro aminopeptidase 1 was increased in haze eyes relative to nonhaze eyes (1.517, P = 0.023). CONCLUSIONS. Corneal haze following corneal inlay surgery is associated with reduction in levels of known inflammatory and immune mediators. These findings represent a starting point for elucidation of pathways involved in corneal haze following synthetic inlay implantation and may enable development of targeted therapies that modulate the haze response.
KW - Corneal inlay
KW - Corneal wound healing
KW - Haze
KW - Refractive surgery
KW - Tear proteomics
UR - http://www.scopus.com/inward/record.url?scp=85063469232&partnerID=8YFLogxK
U2 - 10.1167/iovs.18-25761
DO - 10.1167/iovs.18-25761
M3 - Journal article
C2 - 30821812
AN - SCOPUS:85063469232
SN - 0146-0404
VL - 60
SP - 868
EP - 876
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 4
ER -