Maternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34+ cells

Yvonne K. Kwok, Mary H Y Tang, Ka Wai Helen Law, Cora S. Ngai, Yu Lung Lau, Elizabeth T. Lau

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)

Abstract

Umbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34+ cells derived from human umbilical cord blood. During CD34 + cell enrichment, including maternal plasma or HSA instead of fetal bovine serum (FBS) in the wash buffer, significantly increased the purity and the fold expansion of CD34+ cells. The increase in fold expansion of CD34+ cells was independent of CD34+ cell purity before expansion. With FBS, the mean fold expansion of CD34+ cells and total nucleated cells on day 7 was 9.7 ± 5.5 and 39.7 ± 13.7 respectively. The use of maternal plasma increased the mean fold expansion of CD34+ cells and total nucleated cells on day 7 to 28.2 ± 6.7 and 71.5 ± 15.4 respectively. When HSA was added to wash buffer, the mean fold expansion of CD34+ cells and total nucleated cells were 30.4 ± 10.5 and 83.5 ± 24.8 respectively. No statistical significance was found between using HSA and maternal plasma on total cell and CD34 + cell expansion. We propose that HSA in maternal plasma was responsible for the positive effect on CD34+ cell enrichment and expansion.
Original languageEnglish
Pages (from-to)468-474
Number of pages7
JournalBritish Journal of Haematology
Volume137
Issue number5
DOIs
Publication statusPublished - 1 Jun 2007
Externally publishedYes

Keywords

  • CD34 cells +
  • Haemopoietic primitive cell
  • Human serum albumin
  • Maternal plasma
  • Umbilical cord blood

ASJC Scopus subject areas

  • Hematology

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