Abstract
Umbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34+ cells derived from human umbilical cord blood. During CD34 + cell enrichment, including maternal plasma or HSA instead of fetal bovine serum (FBS) in the wash buffer, significantly increased the purity and the fold expansion of CD34+ cells. The increase in fold expansion of CD34+ cells was independent of CD34+ cell purity before expansion. With FBS, the mean fold expansion of CD34+ cells and total nucleated cells on day 7 was 9.7 ± 5.5 and 39.7 ± 13.7 respectively. The use of maternal plasma increased the mean fold expansion of CD34+ cells and total nucleated cells on day 7 to 28.2 ± 6.7 and 71.5 ± 15.4 respectively. When HSA was added to wash buffer, the mean fold expansion of CD34+ cells and total nucleated cells were 30.4 ± 10.5 and 83.5 ± 24.8 respectively. No statistical significance was found between using HSA and maternal plasma on total cell and CD34 + cell expansion. We propose that HSA in maternal plasma was responsible for the positive effect on CD34+ cell enrichment and expansion.
Original language | English |
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Pages (from-to) | 468-474 |
Number of pages | 7 |
Journal | British Journal of Haematology |
Volume | 137 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 Jun 2007 |
Externally published | Yes |
Keywords
- CD34 cells +
- Haemopoietic primitive cell
- Human serum albumin
- Maternal plasma
- Umbilical cord blood
ASJC Scopus subject areas
- Hematology