Maslinic acid promotes autophagy by disrupting the interaction between Bcl2 and Beclin1 in rat phenochromocytoma PC12 cells.

Xiaoli Dong; Jiaxiao ZHANG; Zhilin ZHOU; Zhennan YE; Jiahao CHEN; Jifan YUAN; Fengjun CAO; Xuanbin WANG; Wenchao LIU; Wenxuan Yu; Xiaohua Li

doi:10.18632/oncotarget.20210

Maslinic acid promotes autophagy by disrupting the interaction between Bcl2 and Beclin1 in rat phenochromocytoma PC12 cells.

Xiaoli Dong, Jiaxiao ZHANG, Zhilin ZHOU, Zhennan YE, Jiahao CHEN, Jifan YUAN, Fengjun CAO, Xuanbin WANG, Wenchao LIU, Wenxuan Yu, Xiaohua Li

Research output: Journal article publication › Journal article › Academic research › peer-review

19 Citations (Scopus)

Abstract

Maslinic acid (2α, 3β-dihydroxyolean-12-en-28-oic acid, MA) was isolated from natural plants and showed anti-cancer activity in rat Pheochromocytoma PC12 cells in our previous studies. We now discover that MA disrupts the interaction between Bcl2 and autophagy scaffold protein Beclin1 in the above cell line, leading to the up-regulation of autophagy. We investigated the effect of MA on the interaction between Bcl2 and Beclin1 by biochemical and biophysical methods in combination with autophagy characterization in the above cell line. Our results suggest that MA may serve as an autophagy activator by directly blocking the Bcl2-Beclin1 interaction to release free Beclin1 required for the recruitment of autophagy positive regulators, implying MA may exert its anti-cancer activity by regulating autophagy.

Original language	English
Article number	PMID: 29088805
Pages (from-to)	74527-74538
Journal	Oncotarget
Volume	8
Issue number	43
DOIs	https://doi.org/10.18632/oncotarget.20210
Publication status	Published - 5 Aug 2017

ASJC Scopus subject areas

Biophysics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.20210

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title = "Maslinic acid promotes autophagy by disrupting the interaction between Bcl2 and Beclin1 in rat phenochromocytoma PC12 cells.",

abstract = "Maslinic acid (2α, 3β-dihydroxyolean-12-en-28-oic acid, MA) was isolated from natural plants and showed anti-cancer activity in rat Pheochromocytoma PC12 cells in our previous studies. We now discover that MA disrupts the interaction between Bcl2 and autophagy scaffold protein Beclin1 in the above cell line, leading to the up-regulation of autophagy. We investigated the effect of MA on the interaction between Bcl2 and Beclin1 by biochemical and biophysical methods in combination with autophagy characterization in the above cell line. Our results suggest that MA may serve as an autophagy activator by directly blocking the Bcl2-Beclin1 interaction to release free Beclin1 required for the recruitment of autophagy positive regulators, implying MA may exert its anti-cancer activity by regulating autophagy.",

author = "Xiaoli Dong and Jiaxiao ZHANG and Zhilin ZHOU and Zhennan YE and Jiahao CHEN and Jifan YUAN and Fengjun CAO and Xuanbin WANG and Wenchao LIU and Wenxuan Yu and Xiaohua Li",

year = "2017",

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doi = "10.18632/oncotarget.20210",

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T1 - Maslinic acid promotes autophagy by disrupting the interaction between Bcl2 and Beclin1 in rat phenochromocytoma PC12 cells.

AU - Dong, Xiaoli

AU - ZHANG, Jiaxiao

AU - ZHOU, Zhilin

AU - YE, Zhennan

AU - CHEN, Jiahao

AU - YUAN, Jifan

AU - CAO, Fengjun

AU - WANG, Xuanbin

AU - LIU, Wenchao

AU - Yu, Wenxuan

AU - Li, Xiaohua

PY - 2017/8/5

Y1 - 2017/8/5

N2 - Maslinic acid (2α, 3β-dihydroxyolean-12-en-28-oic acid, MA) was isolated from natural plants and showed anti-cancer activity in rat Pheochromocytoma PC12 cells in our previous studies. We now discover that MA disrupts the interaction between Bcl2 and autophagy scaffold protein Beclin1 in the above cell line, leading to the up-regulation of autophagy. We investigated the effect of MA on the interaction between Bcl2 and Beclin1 by biochemical and biophysical methods in combination with autophagy characterization in the above cell line. Our results suggest that MA may serve as an autophagy activator by directly blocking the Bcl2-Beclin1 interaction to release free Beclin1 required for the recruitment of autophagy positive regulators, implying MA may exert its anti-cancer activity by regulating autophagy.

AB - Maslinic acid (2α, 3β-dihydroxyolean-12-en-28-oic acid, MA) was isolated from natural plants and showed anti-cancer activity in rat Pheochromocytoma PC12 cells in our previous studies. We now discover that MA disrupts the interaction between Bcl2 and autophagy scaffold protein Beclin1 in the above cell line, leading to the up-regulation of autophagy. We investigated the effect of MA on the interaction between Bcl2 and Beclin1 by biochemical and biophysical methods in combination with autophagy characterization in the above cell line. Our results suggest that MA may serve as an autophagy activator by directly blocking the Bcl2-Beclin1 interaction to release free Beclin1 required for the recruitment of autophagy positive regulators, implying MA may exert its anti-cancer activity by regulating autophagy.

U2 - 10.18632/oncotarget.20210

DO - 10.18632/oncotarget.20210

M3 - Journal article

SN - 1949-2553

VL - 8

SP - 74527

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JO - Oncotarget

JF - Oncotarget

IS - 43

M1 - PMID: 29088805

ER -

Maslinic acid promotes autophagy by disrupting the interaction between Bcl2 and Beclin1 in rat phenochromocytoma PC12 cells.

Abstract

ASJC Scopus subject areas

UN SDGs

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