@article{c4fac42c75814966919951c5611a1244,
title = "Magnetic-Responsive Surface-Enhanced Raman Scattering Platform with Tunable Hot Spot for Ultrasensitive Virus Nucleic Acid Detection",
abstract = "Surface-enhanced Raman scattering (SERS)-based biosensors are promising tools for virus nucleic acid detection. However, it remains challenging for SERS-based biosensors using a sandwiching strategy to detect long-chain nucleic acids such as nucleocapsid (N) gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because the extension of the coupling distance (CD) between the two tethered metallic nanostructures weakens electric field and SERS signals. Herein, we report a magnetic-responsive substrate consisting of heteoronanostructures that controls the CD for ultrasensitive and highly selective detection of the N gene of SARS-CoV-2. Significantly, our findings show that this platform reversibly shortens the CD and enhances SERS signals with a 10-fold increase in the detection limit from 1 fM to 100 aM, compared to those without magnetic modulation. The optical simulation that emulates the CD shortening process confirms the CD-dependent electric field strength and further supports the experimental results. Our study provides new insights into designing a stimuli-responsive SERS-based platform with tunable hot spots for long-chain nucleic acid detection.",
keywords = "DNA flexibility, long-chain nucleic acid detection, magnetic modulation, plasmon coupling distance, surface-enhanced Raman signal",
author = "Bohan Yin and Ho, \{Willis Kwun Hei\} and Qin Zhang and Chuanqi Li and Yingying Huang and Jiaxiang Yan and Hongrong Yang and Jianhua Hao and Wong, \{Siu Hong Dexter\} and Mo Yang",
note = "Funding Information: This work was supported by the Shenzhen-Hong Kong-Macao Science and Technology Plan Project (Category C, SGDX2020110309260000) and the Research Grants Council (RGC) of Hong Kong Collaborative Research Grant (C5110–20GF). S.H.D.W. acknowledges the Start-up Funding (0033912) from the Department of Biomedical Engineering and Start-up Fund for RAPs under the Strategic Hiring Scheme (0035876), the Hong Kong Polytechnic University (PolyU, University Grant Council), for supporting this work. This work was also supported by the National Natural Science Foundation of China (NSFC) (grant nos. 31771077), the Research Grants Council (RGC) of Hong Kong General Research Grant (PolyU 15214619E and PolyU 15210818E), and the Hong Kong Polytechnic University Internal Fund (1-ZE1E). This work was also supported by the University Research Facility in Life Sciences of PolyU. The authors thank Pendy Ho and Hardy Lui from the University Research Facility in Materials Characterization and Device Fabrication at PolyU for guidance in FESEM imaging and AFM imaging. They also thank Chi Hang Chow from the University Research Facility in Chemical and Environmental Analysis at PolyU for his help with Raman Spectroscopy. Funding Information: This work was supported by the Shenzhen-Hong Kong-Macao Science and Technology Plan Project (Category C, SGDX2020110309260000) and the Research Grants Council (RGC) of Hong Kong Collaborative Research Grant (C5110?20GF). S.H.D.W. acknowledges the Start-up Funding (0033912) from the Department of Biomedical Engineering and Start-up Fund for RAPs under the Strategic Hiring Scheme (0035876), the Hong Kong Polytechnic University (PolyU, University Grant Council), for supporting this work. This work was also supported by the National Natural Science Foundation of China (NSFC) (grant nos. 31771077), the Research Grants Council (RGC) of Hong Kong General Research Grant (PolyU 15214619E and PolyU 15210818E), and the Hong Kong Polytechnic University Internal Fund (1-ZE1E). This work was also supported by the University Research Facility in Life Sciences of PolyU. The authors thank Pendy Ho and Hardy Lui from the University Research Facility in Materials Characterization and Device Fabrication at PolyU for guidance in FESEM imaging and AFM imaging. They also thank Chi Hang Chow from the University Research Facility in Chemical and Environmental Analysis at PolyU for his help with Raman Spectroscopy. Publisher Copyright: {\textcopyright} 2022 American Chemical Society",
year = "2022",
month = jan,
day = "26",
doi = "10.1021/acsami.1c21173",
language = "English",
volume = "14",
pages = "4714--4724",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "3",
}