Longitudinal study of adult zebrafish heart regeneration using high frequency echocardiography

Lei Sun, Ching Ling Lien, Qiong Wu, Jin Ho Chang, K. Kirk Shung

Research output: Journal article publicationConference articleAcademic researchpeer-review

1 Citation (Scopus)

Abstract

Understanding heart regeneration in a model system is a highly relevant public health . Coronary heart disease is among the leading of disability and mortality worldwide. Injured hearts caused by myocardial infarction (MI) in decreased cardiac performance and eventually development of heart failure. In contrast to mammals, fully regenerate myocardium after 20% resection and provide an excellent model to study the mechanisms of heart regeneration. It been shown that high-frequency echocardiography is feasible to obtain in vivo structural, and functional information of zebrafish heart [1]. In this research, heart amputated fish (n=12) were to regenerate for 14 days. Real-time B-mode and ventricular pulsed-wave Doppler waveforms acquired daily for 14 days. Measurement on the data was performed to obtain cardiac parameters, such as scar volumes, rates, and E/A ratios. Experimental results show a decrease of the scar volume from 0.05±0.012 to .015±0.004 mm3along the days post amputation, which the replacement of the blood clot by regenerated . The E/A ratio display a sudden increase 0.08±0.05 to 0.31±0.15 immediately after the amputation, followed by a gradual decrease to .18±0.08 at 9dpa. This indicates the continuing recovery ventricular functions, correlating well with the regeneration. Heart rate, however, was not significant change along the regenerative process.
Original languageEnglish
Article number4803315
Pages (from-to)1738-1741
Number of pages4
JournalProceedings - IEEE Ultrasonics Symposium
DOIs
Publication statusPublished - 1 Dec 2008
Event2008 IEEE International Ultrasonics Symposium, IUS 2008 - Beijing, China
Duration: 2 Nov 20085 Nov 2008

Keywords

  • Echocardiography
  • Heart regeneration
  • high Frequency ultrasound
  • Pulsed-wave Doppler
  • Zebrafish

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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