Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine–Shell Nanoprobe

Wai Ki Ricky Wong; Siu Hong Wong; Liming Bian

Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine–Shell Nanoprobe

Wai Ki Ricky Wong, Siu Hong Wong, Liming Bian

Department of Biomedical Engineering

Research output: Journal article publication › Journal article › Academic research › peer-review

Abstract

Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines in vitro. A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.

Original language	English
Article number	6
Pages (from-to)	3778-3783
Number of pages	6
Journal	ACS Biomaterials Science and Engineering
Volume	6
Issue number	7
Publication status	Published - 13 Jul 2020

Keywords

cancer cellular uptake
fluorescence quenching
gold nanoprobes
long-term real-time imaging
oncogenic miRNA

ASJC Scopus subject areas

General Materials Science
General Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

https://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.0c00633

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title = "Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine–Shell Nanoprobe",

abstract = "Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines in vitro. A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.",

keywords = "cancer cellular uptake, fluorescence quenching, gold nanoprobes, long-term real-time imaging, oncogenic miRNA",

author = "Wong, {Wai Ki Ricky} and Wong, {Siu Hong} and Liming Bian",

note = "Funding Information: Project 31570979 was supported by the National Natural Science Foundation of China. The work described in this paper is supported by the General Research Fund grant from the Research Grants Council of Hong Kong (14220716). This work is supported by the Health and Medical Research Fund, the Food and Health Bureau, the Government of the Hong Kong Special Administrative Region (03140056). This work is supported by Shenzhen Science and Technology Innovation Commission Project JCYJ20170307165611557. This research is supported by the Chow Yuk Ho Technology Centre for Innovative Medicine, The Chinese University of Hong Kong. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = jul,

day = "13",

language = "English",

volume = "6",

pages = "3778--3783",

journal = "ACS Biomaterials Science and Engineering",

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AU - Wong, Wai Ki Ricky

AU - Wong, Siu Hong

AU - Bian, Liming

N1 - Funding Information: Project 31570979 was supported by the National Natural Science Foundation of China. The work described in this paper is supported by the General Research Fund grant from the Research Grants Council of Hong Kong (14220716). This work is supported by the Health and Medical Research Fund, the Food and Health Bureau, the Government of the Hong Kong Special Administrative Region (03140056). This work is supported by Shenzhen Science and Technology Innovation Commission Project JCYJ20170307165611557. This research is supported by the Chow Yuk Ho Technology Centre for Innovative Medicine, The Chinese University of Hong Kong. Publisher Copyright: Copyright © 2020 American Chemical Society. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/7/13

Y1 - 2020/7/13

N2 - Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines in vitro. A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.

AB - Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines in vitro. A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.

KW - cancer cellular uptake

KW - fluorescence quenching

KW - gold nanoprobes

KW - long-term real-time imaging

KW - oncogenic miRNA

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M3 - Journal article

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EP - 3783

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

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ER -

Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine–Shell Nanoprobe

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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