Liver-Specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation

Amy P. Chiu, Vincent W. Keng (Corresponding Author)

Research output: Chapter in book / Conference proceedingChapter in an edited book (as author)Academic researchpeer-review


Understanding the complex genetic background of cancers is key in developing effective targeted therapies. The Sleeping Beauty (SB) transposon system is a powerful and unbiased genetic editing tool that can be used for rapid screening of candidate liver cancer driver genes. Manipulating their expression level using a reverse genetic mouse model involving hydrodynamic tail-vein injection delivery can rapidly elucidate the role of these candidate genes in liver cancer tumorigenesis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cancer gene discovery
  • Cancer gene validation
  • Gene delivery
  • Hydrodynamic tail-vein injection
  • Sleeping Beauty transposon system

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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