Liver-Specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation

Amy P. Chiu, Vincent W. Keng (Corresponding Author)

Research output: Chapter in book / Conference proceedingChapter in an edited book (as author)Academic researchpeer-review

Abstract

Understanding the complex genetic background of cancers is key in developing effective targeted therapies. The Sleeping Beauty (SB) transposon system is a powerful and unbiased genetic editing tool that can be used for rapid screening of candidate liver cancer driver genes. Manipulating their expression level using a reverse genetic mouse model involving hydrodynamic tail-vein injection delivery can rapidly elucidate the role of these candidate genes in liver cancer tumorigenesis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-196
Number of pages12
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1907
ISSN (Print)1064-3745

Keywords

  • Cancer gene discovery
  • Cancer gene validation
  • Gene delivery
  • Hydrodynamic tail-vein injection
  • Sleeping Beauty transposon system

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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