Abstract
Voltage sensitive fluorescent dyes have long been used to measure physiological voltages in live cell cultures. However dyes suffer from poor contrast and limited recording duration due to photobleaching. A photostable voltage sensitive cellular label, such as a noble metal nanoparticle, would potentially allow for indefinite recording from neural and other live cell cultures. Noble metals possess an inherent voltage sensitivity: their optical properties depend on their density of free electrons, which can be modulated in an aqueous environment by charging or discharging the double layer capacitance with an applied voltage. This manuscript contains a simple analysis of the expected voltage sensitivity using gold nanospheres and nanoshells in both darkfield and photothermal detection modalities and concludes that high bandwidth voltage measurement is fundamentally achievable.
Original language | English |
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Article number | 718006 |
Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 7180 |
DOIs | |
Publication status | Published - 1 Jun 2009 |
Externally published | Yes |
Event | Photons and Neurons - San Jose, CA, United States Duration: 25 Jan 2009 → 26 Jan 2009 |
Keywords
- Drude
- Gold
- Lorentz
- Mie
- Nanoshell
- Nanosphere
- Particle plasmon resonance
- Voltage sensitivity
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging