Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

D. G. Waugh; J. Lawrence; P. Shukla; C. Chan; I. Hussain; Hau Chung Man; G. C. Smith

doi:10.1117/12.2182432

Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

D. G. Waugh, J. Lawrence, P. Shukla, C. Chan, I. Hussain, Hau Chung Man, G. C. Smith

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

2 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.

Original language	English
Title of host publication	Industrial Laser Applications Symposium, ILAS 2015
Publisher	SPIE
Volume	9657
ISBN (Electronic)	9781628418705
DOIs	https://doi.org/10.1117/12.2182432
Publication status	Published - 1 Jan 2015
Event	4th Industrial Laser Applications Symposium, ILAS 2015 - Kenilworth, United Kingdom Duration: 17 Mar 2015 → 18 Mar 2015

Conference

Conference	4th Industrial Laser Applications Symposium, ILAS 2015
Country/Territory	United Kingdom
City	Kenilworth
Period	17/03/15 → 18/03/15

Keywords

cell viability
CO laser 2
fiber laser
laser surface treatment
MTT
stem cells

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

More information

10.1117/12.2182432

Cite this

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abstract = "Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.",

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Waugh, DG, Lawrence, J, Shukla, P, Chan, C, Hussain, I, Man, HC & Smith, GC 2015, Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. in Industrial Laser Applications Symposium, ILAS 2015. vol. 9657, 96570L, SPIE, 4th Industrial Laser Applications Symposium, ILAS 2015, Kenilworth, United Kingdom, 17/03/15. https://doi.org/10.1117/12.2182432

Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. / Waugh, D. G.; Lawrence, J.; Shukla, P. et al.
Industrial Laser Applications Symposium, ILAS 2015. Vol. 9657 SPIE, 2015. 96570L.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

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AU - Man, Hau Chung

AU - Smith, G. C.

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Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

Abstract

Conference

Keywords

ASJC Scopus subject areas

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