Label-free protein recognition using an aptamer-based impedance measurement assay

Hong Cai, Ming Hung Thomas Lee, I. Ming Hsing

Research output: Journal article publicationJournal articleAcademic researchpeer-review

160 Citations (Scopus)

Abstract

A novel aptamer-based biosensing assay for label-free protein detection and quantification by measuring the change in electrochemical impedance upon protein-aptamer complex formation was demonstrated. Our process starts with a self-assembly of the DNA aptamer on a microfabricated thin film gold electrode, followed by the recognition of the protein binding event via monitoring the interfacial electron transfer resistance with electrochemical impedance spectroscopy. The thrombin binding aptamer (TBA) has high binding affinity and specificity to its target protein and the impedance detection assay shows a reliable and sensitive quantification of thrombin with a detection range of four orders of magnitude and a detection limit of 0.1 nM.
Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume114
Issue number1
DOIs
Publication statusPublished - 30 Mar 2006
Externally publishedYes

Keywords

  • Aptamer
  • Electrochemical impedance spectroscopy
  • Label-free protein detection
  • Thrombin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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