Label-Free DNA Detection Using Etched Tilted Bragg Fiber Grating-Based Biosensor

Abdullah Al Noman; Jitendra Narayan Dash; Md Abdullah Al Maruf; Xin Cheng; Hwa Yam Tam; Changyuan Yu

doi:10.3390/s23167019

Label-Free DNA Detection Using Etched Tilted Bragg Fiber Grating-Based Biosensor

Abdullah Al Noman, Jitendra Narayan Dash, Md Abdullah Al Maruf, Xin Cheng, Hwa Yam Tam, Changyuan Yu

Research output: Journal article publication › Journal article › Academic research › peer-review

6 Citations (Scopus)

Abstract

A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe tilted fiber Bragg grating with a tilt angle of 10°, while the etching has been accomplished with hydrofluoric acid. A composite of polyethylenimine (PEI)/poly(acrylic acid) (PAA) has been thermally deposited on the etched TFBG, followed by immobilization of probe DNA (pDNA) on this deposited layer. The hybridization of pDNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated by fabricating three identical probes and their response has been investigated for cDNA concentration ranging from 0 μM to 3 μM. The maximum sensitivity has been found to be 320 pm/μM, with the detection limit being 0.65 μM. Furthermore, the response of the probes towards non-cDNA has also been investigated in order to establish its specificity.

Original language	English
Article number	7019
Journal	Sensors
Volume	23
Issue number	16
DOIs	https://doi.org/10.3390/s23167019
Publication status	Published - Aug 2023

Keywords

biosensor
DNA
etching
optical fiber sensor
tilted fiber Bragg grating

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

More information

10.3390/s23167019

http://hdl.handle.net/10397/108730

Cite this

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title = "Label-Free DNA Detection Using Etched Tilted Bragg Fiber Grating-Based Biosensor",

abstract = "A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe tilted fiber Bragg grating with a tilt angle of 10°, while the etching has been accomplished with hydrofluoric acid. A composite of polyethylenimine (PEI)/poly(acrylic acid) (PAA) has been thermally deposited on the etched TFBG, followed by immobilization of probe DNA (pDNA) on this deposited layer. The hybridization of pDNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated by fabricating three identical probes and their response has been investigated for cDNA concentration ranging from 0 μM to 3 μM. The maximum sensitivity has been found to be 320 pm/μM, with the detection limit being 0.65 μM. Furthermore, the response of the probes towards non-cDNA has also been investigated in order to establish its specificity.",

keywords = "biosensor, DNA, etching, optical fiber sensor, tilted fiber Bragg grating",

author = "Noman, {Abdullah Al} and Dash, {Jitendra Narayan} and Maruf, {Md Abdullah Al} and Xin Cheng and Tam, {Hwa Yam} and Changyuan Yu",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

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doi = "10.3390/s23167019",

language = "English",

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journal = "Sensors",

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AU - Noman, Abdullah Al

AU - Dash, Jitendra Narayan

AU - Maruf, Md Abdullah Al

AU - Cheng, Xin

AU - Tam, Hwa Yam

AU - Yu, Changyuan

PY - 2023/8

Y1 - 2023/8

N2 - A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe tilted fiber Bragg grating with a tilt angle of 10°, while the etching has been accomplished with hydrofluoric acid. A composite of polyethylenimine (PEI)/poly(acrylic acid) (PAA) has been thermally deposited on the etched TFBG, followed by immobilization of probe DNA (pDNA) on this deposited layer. The hybridization of pDNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated by fabricating three identical probes and their response has been investigated for cDNA concentration ranging from 0 μM to 3 μM. The maximum sensitivity has been found to be 320 pm/μM, with the detection limit being 0.65 μM. Furthermore, the response of the probes towards non-cDNA has also been investigated in order to establish its specificity.

AB - A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe tilted fiber Bragg grating with a tilt angle of 10°, while the etching has been accomplished with hydrofluoric acid. A composite of polyethylenimine (PEI)/poly(acrylic acid) (PAA) has been thermally deposited on the etched TFBG, followed by immobilization of probe DNA (pDNA) on this deposited layer. The hybridization of pDNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated by fabricating three identical probes and their response has been investigated for cDNA concentration ranging from 0 μM to 3 μM. The maximum sensitivity has been found to be 320 pm/μM, with the detection limit being 0.65 μM. Furthermore, the response of the probes towards non-cDNA has also been investigated in order to establish its specificity.

KW - biosensor

KW - DNA

KW - etching

KW - optical fiber sensor

KW - tilted fiber Bragg grating

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U2 - 10.3390/s23167019

DO - 10.3390/s23167019

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AN - SCOPUS:85168741799

SN - 1424-8220

VL - 23

JO - Sensors

JF - Sensors

IS - 16

M1 - 7019

ER -

Label-Free DNA Detection Using Etched Tilted Bragg Fiber Grating-Based Biosensor

Abstract

Keywords

ASJC Scopus subject areas

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