Label-free, disposable fiber-optic biosensors for DNA hybridization detection

Ming Jie Yin, Chuang Wu, Li Yang Shao, Wing Kin Edward Chan, Aping Zhang, Chao Lu, Hwa Yaw Tam

Research output: Journal article publicationJournal articleAcademic researchpeer-review

33 Citations (Scopus)


A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/ DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.
Original languageEnglish
Pages (from-to)1988-1994
Number of pages7
Issue number7
Publication statusPublished - 7 Apr 2013

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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