Label-free, disposable fiber-optic biosensors for DNA hybridization detection

Ming Jie Yin; Chuang Wu; Li Yang Shao; Wing Kin Edward Chan; Aping Zhang; Chao Lu; Hwa Yaw Tam

doi:10.1039/c3an36791f

Label-free, disposable fiber-optic biosensors for DNA hybridization detection

Ming Jie Yin, Chuang Wu, Li Yang Shao, Wing Kin Edward Chan, Aping Zhang, Chao Lu, Hwa Yaw Tam

The Hong Kong Polytechnic University

Research output: Journal article publication › Journal article › Academic research › peer-review

42 Citations (Scopus)

Abstract

A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/ DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.

Original language	English
Pages (from-to)	1988-1994
Number of pages	7
Journal	Analyst
Volume	138
Issue number	7
DOIs	https://doi.org/10.1039/c3an36791f
Publication status	Published - 7 Apr 2013

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/c3an36791f

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title = "Label-free, disposable fiber-optic biosensors for DNA hybridization detection",

abstract = "A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/ DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.",

author = "Yin, {Ming Jie} and Chuang Wu and Shao, {Li Yang} and Chan, {Wing Kin Edward} and Aping Zhang and Chao Lu and Tam, {Hwa Yaw}",

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T1 - Label-free, disposable fiber-optic biosensors for DNA hybridization detection

AU - Yin, Ming Jie

AU - Wu, Chuang

AU - Shao, Li Yang

AU - Chan, Wing Kin Edward

AU - Zhang, Aping

AU - Lu, Chao

AU - Tam, Hwa Yaw

PY - 2013/4/7

Y1 - 2013/4/7

N2 - A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/ DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.

AB - A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/ DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.

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DO - 10.1039/c3an36791f

M3 - Journal article

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VL - 138

SP - 1988

EP - 1994

JO - Analyst

JF - Analyst

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ER -

Label-free, disposable fiber-optic biosensors for DNA hybridization detection

Abstract

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