Abstract
We demonstrate for the first time the utility of nucleic acid aptamers for electrochemical detection of proteins. Highly specific and sensitive label-free detection of the target protein is achieved by combining aptamer-coated magnetic beads and chronopotentiometric stripping measurements of the captured protein (in connection to the intrinsic electroactivity of the protein). Lysozyme has thus been detected selectively in a mixture containing a large excess of six proteins and amino acids (both electroactive and non-electroactive), with a detection limit of 350 fmol (7 nM). While aptamer-based electronic sensors are in their infancy, such devices offer attractive opportunities for electrochemical detection of proteins and for developing proteomic chips.
Original language | English |
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Pages (from-to) | 537-540 |
Number of pages | 4 |
Journal | Electrochemistry Communications |
Volume | 7 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 May 2005 |
Externally published | Yes |
Keywords
- Aptamers
- Bioelectronics
- Proteins
- Stripping potentiometry
ASJC Scopus subject areas
- Electrochemistry