Label-free bioelectronic detection of aptamer-protein interactions

Abdel Nasser Kawde, Marcela C. Rodriguez, Ming Hung Thomas Lee, Joseph Wang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

115 Citations (Scopus)


We demonstrate for the first time the utility of nucleic acid aptamers for electrochemical detection of proteins. Highly specific and sensitive label-free detection of the target protein is achieved by combining aptamer-coated magnetic beads and chronopotentiometric stripping measurements of the captured protein (in connection to the intrinsic electroactivity of the protein). Lysozyme has thus been detected selectively in a mixture containing a large excess of six proteins and amino acids (both electroactive and non-electroactive), with a detection limit of 350 fmol (7 nM). While aptamer-based electronic sensors are in their infancy, such devices offer attractive opportunities for electrochemical detection of proteins and for developing proteomic chips.
Original languageEnglish
Pages (from-to)537-540
Number of pages4
JournalElectrochemistry Communications
Issue number5
Publication statusPublished - 1 May 2005
Externally publishedYes


  • Aptamers
  • Bioelectronics
  • Proteins
  • Stripping potentiometry

ASJC Scopus subject areas

  • Electrochemistry

Cite this