TY - JOUR
T1 - Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines
AU - He, Wan
AU - Chan, Charles Ming Lok
AU - Wong, Sze Chuen Cesar
AU - Au, Thomas Chi Chuen
AU - Ho, Wing Shan
AU - Chan, Amanda Kit Ching
AU - Chan, Andrew Sai Kit
AU - Ma, Brigette Buig Yue
AU - Chan, Anthony Tak Cheung
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Although the Notch pathway has been reported to be activated in colorectal cancer (CRC), limited information is available regarding the expression and role of its ligand, Jagged 2 (JAG2), in CRC. Using immunohistochemistry, the present study demonstrated that JAG2 protein expression may be detected in up to 95% of CRC cases and is 3-fold upregulated in tumor cells compared to surrounding normal tissues. This finding suggests that JAG2 may have a role in the tumorigenicity of CRC. To further investigate the cellular functions of JAG2 expression in CRC, two different small interfering RNAs (siRNAs) were used to downregulate JAG2 expression in CRC cell lines (HCT116, DLD-1 and HT-29). The results indicated that JAG2 knockdown inhibits the motility and invasiveness of CRC cell lines without significantly affecting cell proliferation. These findings implicate JAG2 in promoting aggressiveness of CRC, and lay the foundation for its future development as a therapeutic target for the treatment of CRC.
AB - Although the Notch pathway has been reported to be activated in colorectal cancer (CRC), limited information is available regarding the expression and role of its ligand, Jagged 2 (JAG2), in CRC. Using immunohistochemistry, the present study demonstrated that JAG2 protein expression may be detected in up to 95% of CRC cases and is 3-fold upregulated in tumor cells compared to surrounding normal tissues. This finding suggests that JAG2 may have a role in the tumorigenicity of CRC. To further investigate the cellular functions of JAG2 expression in CRC, two different small interfering RNAs (siRNAs) were used to downregulate JAG2 expression in CRC cell lines (HCT116, DLD-1 and HT-29). The results indicated that JAG2 knockdown inhibits the motility and invasiveness of CRC cell lines without significantly affecting cell proliferation. These findings implicate JAG2 in promoting aggressiveness of CRC, and lay the foundation for its future development as a therapeutic target for the treatment of CRC.
KW - Colorectal cancer
KW - Invasion
KW - Jagged 2
KW - Migration
KW - Notch
UR - http://www.scopus.com/inward/record.url?scp=84995794118&partnerID=8YFLogxK
U2 - 10.3892/ol.2016.5321
DO - 10.3892/ol.2016.5321
M3 - Journal article
SN - 1792-1074
VL - 12
SP - 5193
EP - 5198
JO - Oncology Letters
JF - Oncology Letters
IS - 6
ER -
