Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines

Wan He, Charles Ming Lok Chan, Sze Chuen Cesar Wong, Thomas Chi Chuen Au, Wing Shan Ho, Amanda Kit Ching Chan, Andrew Sai Kit Chan, Brigette Buig Yue Ma, Anthony Tak Cheung Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

5 Citations (Scopus)

Abstract

Although the Notch pathway has been reported to be activated in colorectal cancer (CRC), limited information is available regarding the expression and role of its ligand, Jagged 2 (JAG2), in CRC. Using immunohistochemistry, the present study demonstrated that JAG2 protein expression may be detected in up to 95% of CRC cases and is 3-fold upregulated in tumor cells compared to surrounding normal tissues. This finding suggests that JAG2 may have a role in the tumorigenicity of CRC. To further investigate the cellular functions of JAG2 expression in CRC, two different small interfering RNAs (siRNAs) were used to downregulate JAG2 expression in CRC cell lines (HCT116, DLD-1 and HT-29). The results indicated that JAG2 knockdown inhibits the motility and invasiveness of CRC cell lines without significantly affecting cell proliferation. These findings implicate JAG2 in promoting aggressiveness of CRC, and lay the foundation for its future development as a therapeutic target for the treatment of CRC.
Original languageEnglish
Pages (from-to)5193-5198
Number of pages6
JournalOncology Letters
Volume12
Issue number6
DOIs
Publication statusPublished - 1 Dec 2016

Keywords

  • Colorectal cancer
  • Invasion
  • Jagged 2
  • Migration
  • Notch

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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