Isolation and identification of metabolites of bakuchiol in rats

Pei Le Wang; Feng Xiang Zhang; Zuo Cheng Qiu; Zhi Hong Yao; Man Sau Wong; Xin Sheng Yao; Yi Dai

doi:10.1016/j.fitote.2015.11.006

Isolation and identification of metabolites of bakuchiol in rats

Pei Le Wang, Feng Xiang Zhang, Zuo Cheng Qiu, Zhi Hong Yao, Man Sau Wong, Xin Sheng Yao, Yi Dai

Research output: Journal article publication › Journal article › Academic research › peer-review

19 Citations (Scopus)

Abstract

Bakuchiol, the main active component of Psoralea corylifolia, showed a range of significant pharmacological activities, including antimicrobial, antiinflammatory, reduction of bone loss and estrogenic activities. In this research, 12 metabolites, including 11 new compounds, were isolated from the urine and feces of rats after oral administration of bakuchiol, and their structures were elucidated by extensive spectroscopic analysis. The possible metabolic pathways of bakuchiol in rats were proposed, and a rare bile acid conjugation reaction was found. In addition, bakuchiol and its metabolites M1-M3 were studied for their alkaline phosphatase (ALP) activities on MC3T3-E1 cells and cytotoxicity on HKC-8 cells. The data showed that bakuchiol exerted significant effects on ALP activity of MC3T3-E1 cells and cytotoxicity on HKC-8 cells, while M1-M3 only showed ALP activities at 10- 5M on MC3T3-E1 cells and no cytotoxicity on HKC-8 cells.

Original language	English
Pages (from-to)	31-38
Number of pages	8
Journal	Fitoterapia
Volume	109
DOIs	https://doi.org/10.1016/j.fitote.2015.11.006
Publication status	Published - 1 Mar 2016

Keywords

Bakuchiol
HKC-8 cytotoxicity
MC3T3-E1 cell ALP activity
Metabolites
Psoralea corylifolia

ASJC Scopus subject areas

Pharmacology
Drug Discovery

More information

10.1016/j.fitote.2015.11.006

http://hdl.handle.net/10397/101613

Cite this

@article{f037e243ac5d4b3c9b7930ff99d8d177,

title = "Isolation and identification of metabolites of bakuchiol in rats",

abstract = "Bakuchiol, the main active component of Psoralea corylifolia, showed a range of significant pharmacological activities, including antimicrobial, antiinflammatory, reduction of bone loss and estrogenic activities. In this research, 12 metabolites, including 11 new compounds, were isolated from the urine and feces of rats after oral administration of bakuchiol, and their structures were elucidated by extensive spectroscopic analysis. The possible metabolic pathways of bakuchiol in rats were proposed, and a rare bile acid conjugation reaction was found. In addition, bakuchiol and its metabolites M1-M3 were studied for their alkaline phosphatase (ALP) activities on MC3T3-E1 cells and cytotoxicity on HKC-8 cells. The data showed that bakuchiol exerted significant effects on ALP activity of MC3T3-E1 cells and cytotoxicity on HKC-8 cells, while M1-M3 only showed ALP activities at 10- 5M on MC3T3-E1 cells and no cytotoxicity on HKC-8 cells.",

keywords = "Bakuchiol, HKC-8 cytotoxicity, MC3T3-E1 cell ALP activity, Metabolites, Psoralea corylifolia",

author = "Wang, {Pei Le} and Zhang, {Feng Xiang} and Qiu, {Zuo Cheng} and Yao, {Zhi Hong} and Wong, {Man Sau} and Yao, {Xin Sheng} and Yi Dai",

year = "2016",

month = mar,

day = "1",

doi = "10.1016/j.fitote.2015.11.006",

language = "English",

volume = "109",

pages = "31--38",

journal = "Fitoterapia",

issn = "0367-326X",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Isolation and identification of metabolites of bakuchiol in rats

AU - Wang, Pei Le

AU - Zhang, Feng Xiang

AU - Qiu, Zuo Cheng

AU - Yao, Zhi Hong

AU - Wong, Man Sau

AU - Yao, Xin Sheng

AU - Dai, Yi

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Bakuchiol, the main active component of Psoralea corylifolia, showed a range of significant pharmacological activities, including antimicrobial, antiinflammatory, reduction of bone loss and estrogenic activities. In this research, 12 metabolites, including 11 new compounds, were isolated from the urine and feces of rats after oral administration of bakuchiol, and their structures were elucidated by extensive spectroscopic analysis. The possible metabolic pathways of bakuchiol in rats were proposed, and a rare bile acid conjugation reaction was found. In addition, bakuchiol and its metabolites M1-M3 were studied for their alkaline phosphatase (ALP) activities on MC3T3-E1 cells and cytotoxicity on HKC-8 cells. The data showed that bakuchiol exerted significant effects on ALP activity of MC3T3-E1 cells and cytotoxicity on HKC-8 cells, while M1-M3 only showed ALP activities at 10- 5M on MC3T3-E1 cells and no cytotoxicity on HKC-8 cells.

AB - Bakuchiol, the main active component of Psoralea corylifolia, showed a range of significant pharmacological activities, including antimicrobial, antiinflammatory, reduction of bone loss and estrogenic activities. In this research, 12 metabolites, including 11 new compounds, were isolated from the urine and feces of rats after oral administration of bakuchiol, and their structures were elucidated by extensive spectroscopic analysis. The possible metabolic pathways of bakuchiol in rats were proposed, and a rare bile acid conjugation reaction was found. In addition, bakuchiol and its metabolites M1-M3 were studied for their alkaline phosphatase (ALP) activities on MC3T3-E1 cells and cytotoxicity on HKC-8 cells. The data showed that bakuchiol exerted significant effects on ALP activity of MC3T3-E1 cells and cytotoxicity on HKC-8 cells, while M1-M3 only showed ALP activities at 10- 5M on MC3T3-E1 cells and no cytotoxicity on HKC-8 cells.

KW - Bakuchiol

KW - HKC-8 cytotoxicity

KW - MC3T3-E1 cell ALP activity

KW - Metabolites

KW - Psoralea corylifolia

UR - http://www.scopus.com/inward/record.url?scp=84949658397&partnerID=8YFLogxK

U2 - 10.1016/j.fitote.2015.11.006

DO - 10.1016/j.fitote.2015.11.006

M3 - Journal article

C2 - 26586623

SN - 0367-326X

VL - 109

SP - 31

EP - 38

JO - Fitoterapia

JF - Fitoterapia

ER -

Isolation and identification of metabolites of bakuchiol in rats

Abstract

Keywords

ASJC Scopus subject areas

More information

Other files and links

Fingerprint

Cite this