Isolation and assessment of a highly-active anti-inflammatory exopolysaccharide from mycelial fermentation of a medicinal fungus cs-hk1

Long Qing Li, Ang Xin Song, Wing Tak Wong, Jian Yong Wu (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

1 Citation (Scopus)


The purpose of this work was to fractionate the complex exopolysaccharide (EPS) from a medicinal fungus Ophiocordyceps sinensis Cs-HK1 based on the molecular weight (MW) range and to assess the in vitro anti-inflammatory activity of different EPS fractions in THP-1 cell culture. The lower MW fraction (EPS-LM-1) showed a much higher anti-inflammatory activity. EPS-LM-1 was identified as a heteropolysaccharide consisting of mannose, glucose, and galactose residues with an average MW of 360 kDa. EPS-LM-1 significantly inhibited the lipopolysaccharide-induced inflammatory responses with the effective concentrations for 50% inhibition below 5 μµg/mL on a few major proinflammatory markers. With such a notable in vitro anti-inflammatory activity, EPS-LM-1 is a promising candidate for the development of a new anti-inflammation therapy.

Original languageEnglish
Article number2450
Pages (from-to)1-13
Number of pages13
JournalInternational Journal of Molecular Sciences
Issue number5
Publication statusPublished - 1 Mar 2021


  • Anti-inflammatory activity
  • Exopolysaccharide
  • Fractionation
  • Ophiocordyceps sinensis
  • THP-1 cell culture

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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