Involvement of nucleophosmin/B23 in TPA-induced megakaryocytic differentiation of K562 cells

C. Y. Hsu, Yat Ming Yung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

21 Citations (Scopus)

Abstract

Human myelogenous leukaemia K562 cells were induced to undergo megakaryocytic differentiation by treatment with phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) (20 nM, 24-72 h). The steady-state level of nucleophosmin/B23 mRNA decreased during the TPA-induced differentiation. There was also decrease in the level of cellular nucleophosmin/B23 protein and appearance of its degraded product (25 kDa) during the TPA-induced differentiation. Furthermore, K562/B23 (wild type), K562/D1 (Δ280-294) and K562/D2 (Δ263-294) cells were less, while K562/D3 (Δ244-294) cells were more responsive to TPA-induced differentiation as compared to K562/vector or parental K562 cells. Activation of the ERK/MAPK was observed in parental K562 cells upon TPA treatment (5 nM, 5-30 min). As compared to K562/vector cells, less activation of ERK/MAPK was observed in K562/D2 cells, while ERK/MAPK was highly activated in K562/D3 cells upon TPA treatment. Our results indicate that nucleophosmin/B23 plays an important role in TPA-induced differentiation of K562 cells and the amino acids 244-294 at C-terminal of nucleophosmin/B23 could be an important site for regulation of cellular response to differentiation.
Original languageEnglish
Pages (from-to)1320-1326
Number of pages7
JournalBritish Journal of Cancer
Volume89
Issue number7
DOIs
Publication statusPublished - 6 Oct 2003
Externally publishedYes

Keywords

  • Megakaryocytic differentiation
  • Nucleophosmin/B23
  • TPA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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