Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures

Jian Wen Wang, Li Ping Zheng, Jianyong Wu, Ren Xiang Tan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

88 Citations (Scopus)

Abstract

This work was to characterize the generation of nitric oxide (NO) in Taxus yunnanensis cells exposed to low-energy ultrasound (US) and the signal role of NO in elicitation of plant defense responses and secondary metabolite accumulation. The US sonication (3.5-55.6 mW/cm3at 40 kHz fixed frequency) for 2 min induced a rapid and dose-dependent NO production in the Taxus cell culture, which exhibited a biphasic time course, reaching the first plateau within 1.5 h and the second within 7 h after US sonication. The NO donor sodium nitroprusside (SNP) potentiated US-induced H2O2production and cell death. Inhibition of nitric oxide synthase (NOS) activity by Nω-nitro-l-arginine (l-NNA) or scavenging NO by 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde (PTIO) partially blocked the US-induced H2O2production and cell death. Moreover, the NO inhibitors suppressed US-induced activation of phenylalanine ammonium-lyase (PAL) and accumulation of diterpenoid taxanes (Taxol and baccatin III). These results suggest that NO plays a signal role in the US-induced responses and secondary metabolism activities in the Taxus cells.
Original languageEnglish
Pages (from-to)351-358
Number of pages8
JournalNitric Oxide - Biology and Chemistry
Volume15
Issue number4
DOIs
Publication statusPublished - 1 Dec 2006

Keywords

  • Nitric oxide
  • Oxidative burst
  • Paclitaxel production
  • Phenylalanine ammonium-lyase
  • Taxus yunnanensis
  • Ultrasound

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Clinical Biochemistry
  • Cancer Research

Cite this