Involvement of nitric oxide in elicitor-induced defense responses and secondary metabolism of Taxus chinensis cells

Jian Wen Wang, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

44 Citations (Scopus)

Abstract

This work was to characterize the generation of nitric oxide (NO) in Taxus chinensis cells induced by a fungal elicitor extracted from Fusarium oxysporum mycelium and the signal role of NO in the elicitation of plant defense responses and secondary metabolite accumulation. The fungal elicitor at 10-100 μg/ml (carbohydrate equivalent) induced a rapid and dose-dependent NO production in the Taxus cell culture, which exhibited a biphasic time course, reaching the first plateau within 1 h and the second within 12 h of elicitor treatment. The NO donor sodium nitroprusside potentiated elicitor-induced H2O2production and cell death but had little influence on elicitor-induced membrane K+efflux and H+influx (medium alkalinization). NO inhibitors Nω-nitro-l-arginine and 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide partially blocked the elicitor-induced H2O2production and membrane ion fluxes. Moreover, the NO inhibitors suppressed elicitor-induced activation of phenylalanine ammonium-lyase and accumulation of diterpenoid taxanes (paclitaxel and baccatin III). These results suggest that NO plays a signal role in the elicitor-induced responses and secondary metabolism activities in the Taxus cells.
Original languageEnglish
Pages (from-to)298-306
Number of pages9
JournalNitric Oxide - Biology and Chemistry
Volume11
Issue number4
DOIs
Publication statusPublished - 1 Dec 2004

Keywords

  • Fungal elicitor
  • K /H fluxes + +
  • Nitric oxide
  • Oxidative burst
  • Paclitaxel
  • Taxus cells

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Clinical Biochemistry
  • Cancer Research

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