Abstract
Ginsenoside Rg1 is the main pharmacologically active compound of ginsenosides and has demonstrated pharmacological effects in the cardiovascular system, central nervous system and immune system. The involvement of insulin-like growth factor-I receptor (IGF-IR)-dependent pathway and estrogen receptor (ER)-dependent pathway in the biological effect of ginsenoside Rg1 have been demonstrated in our previous study. The present study tested the hypothesis that the protective effects of Rg1 against Aβ25-35- induced toxicity involved activation of the IGF-IR and ER signaling pathways in PC12 cells. Treatment with Aβ25-35decreased the cell viability in a dose-dependent manner in PC12 cells. Rg1 pretreatment resulted in an enhancement of survival and the maximum protection occurred at the concentration of 1 μM. Co-treatment with IGF-IR antagonist JB-1 or ER antagonist ICI182,780 could completely block the protective effect of Rg1. The decreased Bcl-2 mRNA expression induced by Aβ25-35could be restored by Rg1 pretreatment. Rg1 pretreatment could also restore the decreased mitochondrial membrane potential induced by Aβ25-35and these effects could be completely blocked by JB-1 or ICI182,780. In addition, Rg1 treatment alone could significantly increase the phosphorylation level of MEK and ERK in a time-dependent manner and the functional transactivation of ERα in PC12 cells. The functional transactivation of ERα by Rg1 could be completely blocked by JB-1 or ICI182,780. Taken together, our results suggest that IGF-IR and ER signaling pathways might be involved in the protective effect of Rg1 against Aβ25-35-induced toxicity in PC12 cells.
Original language | English |
---|---|
Pages (from-to) | 1065-1071 |
Number of pages | 7 |
Journal | Neurochemistry International |
Volume | 62 |
Issue number | 8 |
DOIs | |
Publication status | Published - 1 Jun 2013 |
Keywords
- β-Amyloid peptide
- Estrogen receptor
- Ginsenoside Rg1
- Insulin-like growth factor-I receptor
- PC12 cells
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Cell Biology