Involvement of IGF-I receptor and estrogen receptor pathways in the protective effects of ginsenoside Rg1 against Aβ25-35-induced toxicity in PC12 cells

Wen Fang Chen, Li Ping Zhou, Lei Chen, Lin Wu, Quan Gui Gao, Man Sau Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)

Abstract

Ginsenoside Rg1 is the main pharmacologically active compound of ginsenosides and has demonstrated pharmacological effects in the cardiovascular system, central nervous system and immune system. The involvement of insulin-like growth factor-I receptor (IGF-IR)-dependent pathway and estrogen receptor (ER)-dependent pathway in the biological effect of ginsenoside Rg1 have been demonstrated in our previous study. The present study tested the hypothesis that the protective effects of Rg1 against Aβ25-35- induced toxicity involved activation of the IGF-IR and ER signaling pathways in PC12 cells. Treatment with Aβ25-35decreased the cell viability in a dose-dependent manner in PC12 cells. Rg1 pretreatment resulted in an enhancement of survival and the maximum protection occurred at the concentration of 1 μM. Co-treatment with IGF-IR antagonist JB-1 or ER antagonist ICI182,780 could completely block the protective effect of Rg1. The decreased Bcl-2 mRNA expression induced by Aβ25-35could be restored by Rg1 pretreatment. Rg1 pretreatment could also restore the decreased mitochondrial membrane potential induced by Aβ25-35and these effects could be completely blocked by JB-1 or ICI182,780. In addition, Rg1 treatment alone could significantly increase the phosphorylation level of MEK and ERK in a time-dependent manner and the functional transactivation of ERα in PC12 cells. The functional transactivation of ERα by Rg1 could be completely blocked by JB-1 or ICI182,780. Taken together, our results suggest that IGF-IR and ER signaling pathways might be involved in the protective effect of Rg1 against Aβ25-35-induced toxicity in PC12 cells.
Original languageEnglish
Pages (from-to)1065-1071
Number of pages7
JournalNeurochemistry International
Volume62
Issue number8
DOIs
Publication statusPublished - 1 Jun 2013

Keywords

  • β-Amyloid peptide
  • Estrogen receptor
  • Ginsenoside Rg1
  • Insulin-like growth factor-I receptor
  • PC12 cells

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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