Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis

X. Y. Wang, Xia Guo, W. L. Lam, J. C.Y. Cheng

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

To investigate the temporal and spatial distribution of calcitonin gene-related peptide (CGRP) positive nerve fibers during the consolidation stage of distraction osteogenesis (DO), twenty New Zealand white rabbits were used for unilateral DO. Distraction started at the 7thday after osteotomy with 1 mm of length per day. The lengthening lasted for 10 days. Four rabbits were sacrificed at each of the following time point at 7-day, 14-day, 21-day, 28-day and 42-day post-distraction. The entire distraction callus and 2 mm of neighboring cortical bone on the proximal and distal sides was removed en bloc for assessments. The results showed that CGRP-positive nerve fibers were observed in the periosteal, intercortical and endocortical regions at 7-day post-distraction. After that, CGRP-positive nerve fibers were mainly found in the fibrous tissue of periosteal region and bone marrow of bony callus. Moreover, the density of CGRP innervations was significantly higher at 21-day post-distraction than that of other time point. In conclusion, the observed spatial and temporal distribution of CGRP-positive nerve fibers during consolidation stage of DO suggested CGRP innervation is associated with DO.
Original languageEnglish
Title of host publication6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages848-851
Number of pages4
Volume31 IFMBE
DOIs
Publication statusPublished - 22 Oct 2010
Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore
Duration: 1 Aug 20106 Aug 2010

Conference

Conference6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
CountrySingapore
CitySingapore
Period1/08/106/08/10

Keywords

  • bone
  • CGRP-positive nerve fibers
  • tibial lengthening

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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