Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis

X. Y. Wang; Xia Guo; W. L. Lam; J. C.Y. Cheng

doi:10.1007/978-3-642-14515-5_216

Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis

X. Y. Wang, Xia Guo, W. L. Lam, J. C.Y. Cheng

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

To investigate the temporal and spatial distribution of calcitonin gene-related peptide (CGRP) positive nerve fibers during the consolidation stage of distraction osteogenesis (DO), twenty New Zealand white rabbits were used for unilateral DO. Distraction started at the 7thday after osteotomy with 1 mm of length per day. The lengthening lasted for 10 days. Four rabbits were sacrificed at each of the following time point at 7-day, 14-day, 21-day, 28-day and 42-day post-distraction. The entire distraction callus and 2 mm of neighboring cortical bone on the proximal and distal sides was removed en bloc for assessments. The results showed that CGRP-positive nerve fibers were observed in the periosteal, intercortical and endocortical regions at 7-day post-distraction. After that, CGRP-positive nerve fibers were mainly found in the fibrous tissue of periosteal region and bone marrow of bony callus. Moreover, the density of CGRP innervations was significantly higher at 21-day post-distraction than that of other time point. In conclusion, the observed spatial and temporal distribution of CGRP-positive nerve fibers during consolidation stage of DO suggested CGRP innervation is associated with DO.

Original language	English
Title of host publication	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages	848-851
Number of pages	4
Volume	31 IFMBE
DOIs	https://doi.org/10.1007/978-3-642-14515-5_216
Publication status	Published - 22 Oct 2010
Event	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore Duration: 1 Aug 2010 → 6 Aug 2010

Conference

Conference	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Country/Territory	Singapore
City	Singapore
Period	1/08/10 → 6/08/10

Keywords

bone
CGRP-positive nerve fibers
tibial lengthening

ASJC Scopus subject areas

Bioengineering
Biomedical Engineering

More information

10.1007/978-3-642-14515-5_216

Cite this

Wang, X. Y., Guo, X., Lam, W. L., & Cheng, J. C. Y. (2010). Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech (Vol. 31 IFMBE, pp. 848-851) https://doi.org/10.1007/978-3-642-14515-5_216

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title = "Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis",

abstract = "To investigate the temporal and spatial distribution of calcitonin gene-related peptide (CGRP) positive nerve fibers during the consolidation stage of distraction osteogenesis (DO), twenty New Zealand white rabbits were used for unilateral DO. Distraction started at the 7thday after osteotomy with 1 mm of length per day. The lengthening lasted for 10 days. Four rabbits were sacrificed at each of the following time point at 7-day, 14-day, 21-day, 28-day and 42-day post-distraction. The entire distraction callus and 2 mm of neighboring cortical bone on the proximal and distal sides was removed en bloc for assessments. The results showed that CGRP-positive nerve fibers were observed in the periosteal, intercortical and endocortical regions at 7-day post-distraction. After that, CGRP-positive nerve fibers were mainly found in the fibrous tissue of periosteal region and bone marrow of bony callus. Moreover, the density of CGRP innervations was significantly higher at 21-day post-distraction than that of other time point. In conclusion, the observed spatial and temporal distribution of CGRP-positive nerve fibers during consolidation stage of DO suggested CGRP innervation is associated with DO.",

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note = "6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech ; Conference date: 01-08-2010 Through 06-08-2010",

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Wang, XY, Guo, X, Lam, WL & Cheng, JCY 2010, Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis. in 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. vol. 31 IFMBE, pp. 848-851, 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech, Singapore, Singapore, 1/08/10. https://doi.org/10.1007/978-3-642-14515-5_216

Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis. / Wang, X. Y.; Guo, Xia; Lam, W. L. et al.
6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. Vol. 31 IFMBE 2010. p. 848-851.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

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AU - Wang, X. Y.

AU - Guo, Xia

AU - Lam, W. L.

AU - Cheng, J. C.Y.

PY - 2010/10/22

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N2 - To investigate the temporal and spatial distribution of calcitonin gene-related peptide (CGRP) positive nerve fibers during the consolidation stage of distraction osteogenesis (DO), twenty New Zealand white rabbits were used for unilateral DO. Distraction started at the 7thday after osteotomy with 1 mm of length per day. The lengthening lasted for 10 days. Four rabbits were sacrificed at each of the following time point at 7-day, 14-day, 21-day, 28-day and 42-day post-distraction. The entire distraction callus and 2 mm of neighboring cortical bone on the proximal and distal sides was removed en bloc for assessments. The results showed that CGRP-positive nerve fibers were observed in the periosteal, intercortical and endocortical regions at 7-day post-distraction. After that, CGRP-positive nerve fibers were mainly found in the fibrous tissue of periosteal region and bone marrow of bony callus. Moreover, the density of CGRP innervations was significantly higher at 21-day post-distraction than that of other time point. In conclusion, the observed spatial and temporal distribution of CGRP-positive nerve fibers during consolidation stage of DO suggested CGRP innervation is associated with DO.

AB - To investigate the temporal and spatial distribution of calcitonin gene-related peptide (CGRP) positive nerve fibers during the consolidation stage of distraction osteogenesis (DO), twenty New Zealand white rabbits were used for unilateral DO. Distraction started at the 7thday after osteotomy with 1 mm of length per day. The lengthening lasted for 10 days. Four rabbits were sacrificed at each of the following time point at 7-day, 14-day, 21-day, 28-day and 42-day post-distraction. The entire distraction callus and 2 mm of neighboring cortical bone on the proximal and distal sides was removed en bloc for assessments. The results showed that CGRP-positive nerve fibers were observed in the periosteal, intercortical and endocortical regions at 7-day post-distraction. After that, CGRP-positive nerve fibers were mainly found in the fibrous tissue of periosteal region and bone marrow of bony callus. Moreover, the density of CGRP innervations was significantly higher at 21-day post-distraction than that of other time point. In conclusion, the observed spatial and temporal distribution of CGRP-positive nerve fibers during consolidation stage of DO suggested CGRP innervation is associated with DO.

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T2 - 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech

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Wang XY, Guo X, Lam WL, Cheng JCY. Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. Vol. 31 IFMBE. 2010. p. 848-851 doi: 10.1007/978-3-642-14515-5_216

Involvement of CGRP-positive nerve fibers in consolidation stage of distraction osteogenesis

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