Investigation on ultrasound assisted extraction of saikosaponins from Radix Bupleuri

Shuna Zhao, Kin Chor Kwok, Hanhua Liang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

96 Citations (Scopus)

Abstract

Ultrasound assisted extraction (UAE) of saikosaponins from Radix Bupleuri (Bupleurum Chinense DC) was studied. The effects of various factors such as time (t), temperature (T), ultrasound power (I), particle size (S), solvent to solid ratio (R) and solvent concentration (C) on the yield of target components were investigated. The experimental results indicated that the extraction by UAE is six times faster than those by conventional thermal extraction. It is also found that fast extraction rate was obtained in the first 30 min of sonication. Prolonged sonication did not give a proportional increase in the yield. For the effect of ultrasound power, the maximum yield was obtained at the power level of around 21 W. Scanning electron microscope (SEM) images of the plant cells after UAE treatment were obtained to provide visual evidence of the sonication effect. The effects of particle size, solvent to solid ratio and solvent concentration on the yield are also discussed. The preliminary optimum conditions for UAE of saikosaponins were found at time of 30 min, temperature of 80 °C, power of 21 W, particle size <0.3 mm, solvent to solid ratio of 25 ml/g and solvent concentration of 50%.
Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalSeparation and Purification Technology
Volume55
Issue number3
DOIs
Publication statusPublished - 1 Jul 2007

Keywords

  • Extraction
  • Radix Bupleuri
  • Saikosaponins
  • Ultrasound

ASJC Scopus subject areas

  • Filtration and Separation

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