Investigation of antibiofilm activity, antibacterial activity, and mechanistic studies of an amphiphilic peptide against Acinetobacter baumannii

Ping Zeng, Lanhua Yi, Jiangtao Xu, Wei Gao, Chen Xu, Sheng Chen (Corresponding Author), Kin Fai Chan (Corresponding Author), Kwok Yin Wong (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)

Abstract

Biofilm-producing pathogens, such as Acinetobacter baumannii, have aroused escalating attention. Because these bacteria could secrete mixture with close-knit architecture and complicated components to resist traditional antibiotics. Here, we reported an amphiphilic peptide denoted as zp3 (GIIAGIIIKIKK-NH2), which showed favorable bioactivity against Acinetobacter baumannii ATCC 19606 (minimal inhibitory concentration, MIC = 4 μM) and low cytotoxicity to mammalian cells Vero (half maximal inhibitory concentration, IC50 > 100 μM). Importantly, zp3 could inhibit the formation of biofilm at micromole level and eliminate around 50% preformed biofilm at 32 μM after 6 h treatment. This peptide was able to bind with biofilm while maintaining a helical structure in a mimic biofilm-rich environment. In vivo test demonstrated that zp3 rescued 33.3% of larvae after 48 h infection and reduced 1 log live bacteria inside the animal body after 6 h treatment. The bactericidal mode for zp3 was attributed to the combination of influencing ions balance at low concentration and inducing permeability alteration and pore formation on the Acinetobacter baumannii membrane at high concentration. Application on medical textiles also proved that zp3 could perform a good antibacterial activity in practice.

Original languageEnglish
Article number183600
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1863
Issue number6
DOIs
Publication statusPublished - 1 Jun 2021

Keywords

  • Acinetobacter baumannii
  • Antibiofilm
  • Antimicrobial peptide
  • Medical textile
  • Membrane potential and permeability

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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