Intracellular uptake and release of poly(ethyleneimine)-co-poly(methyl methacrylate) nanoparticle/pDNA complexes for gene delivery

Min Feng, Daniel Lee, Pei Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

44 Citations (Scopus)

Abstract

Our previous studies demonstrated that cationic nanoparticles composed of well-defined poly(methyl methacrylate) (PMMA) cores surrounded by a hairly poly(ethyleneimine) (PEI) shells have comparative advantages over the PEI system for gene delivery. In this study, we focused on the intracellular uptake and release of PEI-PMMA nanoparticle/pDNA complexes. The behavior of the nanoparticle/pDNA complexes in recipient cells was monitored by using confocal laser scanning microscopy. We found that the nanoparticle/pDNA complexes were internalized very effectively by endocytosis. In the recipient cells the nanoparticles were found localized in the cytoplasm. At the same time, the pDNA carried by the nanoparticles successfully detached from the nanoparticles and localized in the nucleus of the HeLa cells.
Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalInternational Journal of Pharmaceutics
Volume311
Issue number1-2
DOIs
Publication statusPublished - 27 Mar 2006

Keywords

  • Cationic PEI-PMMA nanoparticles
  • Confocal laser scanning microscopy
  • Intracellular behavior
  • Nanoparticle/pDNA complexes

ASJC Scopus subject areas

  • Pharmaceutical Science

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