This study aimed to explore how a three-dimensional (3D) map affects the acquisition of survey knowledge in complex virtual environments (VEs). Subject navigated in virtual and real subway stations, where the virtual station was augmented with either a 3D floor map, a 3D building map, or neither. After subject's navigation in the station, they made the judgment of spatial relative directions between objects. The analyses of response time showed that participants responded faster when they were assisted by the 3D floor or building map than without a map aid in virtual stations. There was no significant difference between survey knowledge acquired in the virtual station augmented with the 3D floor or building map and in the real station. Participants with the 3D floor map performed best in spatial judgments. Implications of these findings to a 3D map design were discussed.