Integrating clinical data and tear proteomics to assess efficacy, ocular surface status, and biomarker response after orthokeratology lens wear

Jimmy S.H. Tse, Jimmy K.W. Cheung, Gigi T.K. Wong, Thomas C. Lam, Kai Yip Choi, Katherine H.Y. So, Christie D.M. Lam, Andes Y.H. Sze, Angel C.K. Wong, Gigi M.C. Yee, Henry H.L. Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)


Purpose: This study evaluated the efficacy and ocular surface status of Breath-O Correct, novel orthokeratology (OK) lenses, worn overnight for 3 months. Lens-induced changes in the tear proteome were evaluated. 

Methods: Thirty-one subjects, aged 19 to 26 years with refractive error from −1.00 to −5.00 D, were randomly assigned 1:1 to the treatment or control group. Refrac-tion, visual acuity, corneal integrity, biomechanics and endothelial health, ocular surface changes, and subjective symptoms were assessed at the baseline, one-month, and three-month visits. The tear proteome was characterized over time using sequential window acquisition of all theoretical ion spectra mass spectrometry. 

Results: Lenses improved uncorrected visual acuity and reduced spherical powers with similar efficacy to other OK lenses. Significant reductions (P < 0.05) in corneal hysteresis (11.12 ± 1.12 to 10.38 ± 1.36 mm Hg) and corneal resistance factor (11.06 ± 1.32 to 9.90 ± 1.45 mm Hg) were observed in the treatment group after one month of lens wear, whereas other assessed factors remained unchanged. Thirteen and eight differentially expressed proteins were found after one month and three months of lens wear, respectively. Two proteins (proline-rich protein 27 and immunoglobulin V regions) were differentially expressed at both visits. 

Conclusions: Over a three-month period, Breath-O Correct lenses were overall safe, well tolerated, efficacious in refractive power reduction, and comparable with other OK lenses. Furthermore, their use caused only minor noninflammatory protein expression changes in the tear proteome.

 Translational Relevance: This study investigated the safety of orthokeratology contact lenses on the ocular surface in molecular aspects and standard clinical parameters.

Original languageEnglish
Article number18
Number of pages13
JournalTranslational Vision Science and Technology
Issue number11
Publication statusPublished - Sept 2021


  • Corneal biomechanics
  • Elastic contact lenses
  • Myopia
  • Orthokeratology
  • Proteomics

ASJC Scopus subject areas

  • Biomedical Engineering
  • Ophthalmology


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