Insulin-like growth factor-I stimulates renal 1,25- dihydroxycholecalciferol synthesis in old rats fed a low calcium diet

Man Sau Wong, Vrishali A. Tembe, Murray J. Favus

Research output: Journal article publicationJournal articleAcademic researchpeer-review

22 Citations (Scopus)

Abstract

The adaptive increase in renal proximal tubule 25-hydroxyvitamin D-α- hydroxylase activity (1-OHase) during dietary calcium restriction is mediated by an increase in parathyroid hormone (PTH) and is inhibited by aging. Recent studies in mature (3-4 mo) rats demonstrated that insulin-like growth factor- I (IGF-I) restored stimulation of renal 1,25-dihydroxycholecalciferol [1,25(OH)2D3] production by low phosphorus diet (LPD), another major stimulus of 1-OHase. These studies were designed to determine whether IGF-I stimulates 1-OHase during low calcium intake in old rats. Male rats were fed a normal calcium diet (NCD, 6 g Ca/kg diet) or low calcium diet (LCD, 0.2g Ca/kg diet) for 14 d, and recombinant human IGF-I [rhlGF-I, 1.4 mg/(24h 160 kg body wt)] or vehicle was administrated via miniosmotic pump for 72 h before killing. In 4-mo-old male Sprague-Dawley rats LCD increased in vitro renal 1-OHase activity in the presence but not in the absence of rhlGF-I. LCD increased in vitro 1-OHase activity in young (1-mo-old) but not old (24-mo- old) male Fischer 344 rats. RhlGF-I increased 1-OHase activity in 24 mo-old rats fed LCD to levels that were not different from those in 1-mo-old rats fed LCD. The results indicate that the adaptive increase in 1-OHase activity due to a LCD is lost by 4 mo in rats and can be restored by pharmacologic doses of rhlGF-I.
Original languageEnglish
Pages (from-to)1147-1152
Number of pages6
JournalJournal of Nutrition
Volume130
Issue number5
Publication statusPublished - 11 May 2000

Keywords

  • 1,25-dihydroxycholecalciferol
  • Aging
  • Kidney
  • Low calcium diet
  • Rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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