Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

Wing K. Tang; Chung H. Chui; Sarwat Fatima; Stanton H.L. Kok; Kai C. Pak; Tian M. Ou; Kin S. Hui; Mei M. Wong; John Wong; Simon Law; S. W. Tsao; King Y. Lam; Philip S.L. Beh; Gopesh Srivastava; Kwok P. Ho; Albert S.C. Chan; Cheuk On Tang

doi:10.3892/ijmm.19.6.953

Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

Wing K. Tang, Chung H. Chui, Sarwat Fatima, Stanton H.L. Kok, Kai C. Pak, Tian M. Ou, Kin S. Hui, Mei M. Wong, John Wong, Simon Law, S. W. Tsao, King Y. Lam, Philip S.L. Beh, Gopesh Srivastava, Kwok P. Ho, Albert S.C. Chan, Cheuk On Tang

Research output: Journal article publication › Journal article › Academic research › peer-review

28 Citations (Scopus)

Abstract

Previous studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongenicity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS50of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 μg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.

Original language	English
Pages (from-to)	953-960
Number of pages	8
Journal	International Journal of Molecular Medicine
Volume	19
Issue number	6
DOIs	https://doi.org/10.3892/ijmm.19.6.953
Publication status	Published - 1 Jun 2007

Keywords

Esophageal squamous cell carcinoma
Oncogenic expression
Telomerase

ASJC Scopus subject areas

Genetics

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3892/ijmm.19.6.953

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Tang, W. K., Chui, C. H., Fatima, S., Kok, S. H. L., Pak, K. C., Ou, T. M., Hui, K. S., Wong, M. M., Wong, J., Law, S., Tsao, S. W., Lam, K. Y., Beh, P. S. L., Srivastava, G., Ho, K. P., Chan, A. S. C., & Tang, C. O. (2007). Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma. International Journal of Molecular Medicine, 19(6), 953-960. https://doi.org/10.3892/ijmm.19.6.953

@article{a0366d1d03724b8985cb0ab3eac7ee9f,

title = "Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma",

abstract = "Previous studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongenicity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS50of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 μg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.",

keywords = "Esophageal squamous cell carcinoma, Oncogenic expression, Telomerase",

author = "Tang, {Wing K.} and Chui, {Chung H.} and Sarwat Fatima and Kok, {Stanton H.L.} and Pak, {Kai C.} and Ou, {Tian M.} and Hui, {Kin S.} and Wong, {Mei M.} and John Wong and Simon Law and Tsao, {S. W.} and Lam, {King Y.} and Beh, {Philip S.L.} and Gopesh Srivastava and Ho, {Kwok P.} and Chan, {Albert S.C.} and Tang, {Cheuk On}",

year = "2007",

month = jun,

day = "1",

doi = "10.3892/ijmm.19.6.953",

language = "English",

volume = "19",

pages = "953--960",

journal = "International Journal of Molecular Medicine",

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Tang, WK, Chui, CH, Fatima, S, Kok, SHL, Pak, KC, Ou, TM, Hui, KS, Wong, MM, Wong, J, Law, S, Tsao, SW, Lam, KY, Beh, PSL, Srivastava, G, Ho, KP, Chan, ASC & Tang, CO 2007, 'Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma', International Journal of Molecular Medicine, vol. 19, no. 6, pp. 953-960. https://doi.org/10.3892/ijmm.19.6.953

Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma. / Tang, Wing K.; Chui, Chung H.; Fatima, Sarwat et al.
In: International Journal of Molecular Medicine, Vol. 19, No. 6, 01.06.2007, p. 953-960.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

AU - Tang, Wing K.

AU - Chui, Chung H.

AU - Fatima, Sarwat

AU - Kok, Stanton H.L.

AU - Pak, Kai C.

AU - Ou, Tian M.

AU - Hui, Kin S.

AU - Wong, Mei M.

AU - Wong, John

AU - Law, Simon

AU - Tsao, S. W.

AU - Lam, King Y.

AU - Beh, Philip S.L.

AU - Srivastava, Gopesh

AU - Ho, Kwok P.

AU - Chan, Albert S.C.

AU - Tang, Cheuk On

PY - 2007/6/1

Y1 - 2007/6/1

N2 - Previous studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongenicity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS50of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 μg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.

AB - Previous studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongenicity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS50of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 μg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.

KW - Esophageal squamous cell carcinoma

KW - Oncogenic expression

KW - Telomerase

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U2 - 10.3892/ijmm.19.6.953

DO - 10.3892/ijmm.19.6.953

M3 - Journal article

C2 - 17487429

SN - 1107-3756

VL - 19

SP - 953

EP - 960

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

IS - 6

ER -

Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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