Abstract
The c-fos transcriptional factor forms an activator protein-1 (AP-1) complex with proteins from the Jun family, which plays an important role in the central nervous system. The responses of AP-1 transcriptional factors induced by kainic acid (KA) treatment have been well studied, although the transcriptional regulation of these KA-induced factors has not been clearly characterized. To investigate the role of different stimuli in controlling of the splicing of c-fos mRNA, we performed reverse transcriptional polymerase chain reaction. The results showed that spliced and unspliced c-fos is present in rat brain following KA treatment and in lipopolysaccharide (LPS)-treated primary mouse cortical brain cell cultures. Furthermore, tyrosine kinase and protein phosphatase inhibitors alter the preponderance of c-fos transcripts following LPS treatment.
Original language | English |
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Pages (from-to) | 17-20 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 305 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jun 2001 |
Externally published | Yes |
Keywords
- Gene regulation
- Kainic acid
- Phosphorylation
- Primary cell culture
- Seizure
- Transcription factors
ASJC Scopus subject areas
- General Neuroscience