Induction of unspliced c-fos messenger RNA in rodent brain by kainic acid and lipopolysaccharide

Zhehui Feng, Ling Yuan Kong, Qiping Qi, Shu Leong Ho, Ningyi Tiao, Guoying Bing, Yifan Han

Research output: Journal article publicationJournal articleAcademic researchpeer-review

2 Citations (Scopus)

Abstract

The c-fos transcriptional factor forms an activator protein-1 (AP-1) complex with proteins from the Jun family, which plays an important role in the central nervous system. The responses of AP-1 transcriptional factors induced by kainic acid (KA) treatment have been well studied, although the transcriptional regulation of these KA-induced factors has not been clearly characterized. To investigate the role of different stimuli in controlling of the splicing of c-fos mRNA, we performed reverse transcriptional polymerase chain reaction. The results showed that spliced and unspliced c-fos is present in rat brain following KA treatment and in lipopolysaccharide (LPS)-treated primary mouse cortical brain cell cultures. Furthermore, tyrosine kinase and protein phosphatase inhibitors alter the preponderance of c-fos transcripts following LPS treatment.
Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalNeuroscience Letters
Volume305
Issue number1
DOIs
Publication statusPublished - 1 Jun 2001
Externally publishedYes

Keywords

  • Gene regulation
  • Kainic acid
  • Phosphorylation
  • Primary cell culture
  • Seizure
  • Transcription factors

ASJC Scopus subject areas

  • Neuroscience(all)

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