Induction of HL-60 apoptosis by ethyl acetate extract of Cordyceps sinensis fungal mycelium

Qiaoxia Zhang, Jianyong Wu, Zongding Hu, Duan Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

49 Citations (Scopus)

Abstract

The cultivated mycelium of a Cordyceps sinensis (Cs) fungus was sequentially extracted by petroleum ether, ethyl acetate (EtOAc), ethanol and water. The EtOAc extract showed the most potent cytotoxic effect against the proliferation of human premyelocytic leukemia cell HL-60, with an ED50≤ 25 μg/ml for 2-day treatment. The EtOAc extract induced the characteristic apoptotic symptoms in the HL-60 cells, DNA fragmentation and chromatin condensation, occurring within 6-8 h of treatment at a dose of 200 μg/ml. The activation of caspase-3 and the specific proteolytic cleavage of poly ADP-ribose polymerase were detected during the course of apoptosis induction. These results suggest that the Cs mycelium extract inhibited the cancer cell proliferation by inducing cell apoptosis.
Original languageEnglish
Pages (from-to)2911-2919
Number of pages9
JournalLife Sciences
Volume75
Issue number24
DOIs
Publication statusPublished - 29 Oct 2004

Keywords

  • Apoptosis
  • Cordyceps sinensis
  • Cytotoxicity
  • HL-60
  • Mycelium extract

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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