Induction of antitumor immunity in mice by the combination of nanoparticle-based photothermolysis and anti-PD-1 checkpoint inhibition

Qizhen Cao, Wanqin Wang, Min Zhou, Qian Huang, Xiaoxia Wen, Jun Zhao, Sixiang Shi, Ku Geng, Fenge Li, Hiroto Hatakeyama, Chunyu Xu, David Piwnica-Worms, Weiyi Peng, Dapeng Zhou, Anil K. Sood, Chun Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

8 Citations (Scopus)


Generation of durable tumor-specific immune response without isolation and expansion of dendritic cells or T cells ex vivo remains a challenge. In this study, we investigated the impact of nanoparticle-mediated photothermolysis in combination with checkpoint inhibition on the induction of systemic antitumor immunity. Photothermolysis based on near-infrared light-absorbing copper sulfide nanoparticles and 15-ns laser pulses combined with the immune checkpoint inhibitor anti-PD-1 antibody (αPD-1) increased tumor infiltration by antigen-presenting cells and CD8-positive T lymphocytes in the B16-OVA mouse model. Moreover, combined photothermolysis, polymeric conjugate of the Toll-like receptor 9 agonist CpG, and αPD-1 significantly prolonged mouse survival after re-inoculation of tumor cells at a distant site compared to individual treatments alone in the poorly immunogenic syngeneic ID8-ip1-Luc ovarian tumor model. Thus, photothermolysis is a promising interventional technique that synergizes with Toll-like receptor 9 agonists and immune checkpoint inhibitors to enhance the abscopal effect in tumors.

Original languageEnglish
Article number102169
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Early online date12 Feb 2020
Publication statusPublished - Apr 2020
Externally publishedYes


  • Abscopal effect
  • Checkpoint inhibitor
  • CuS nanoparticle
  • Photothermolysis
  • Pulsed laser

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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