In vivo fluorescence imaging of threadlike tissues (Bonghan ducts) inside lymphatic vessels with nanoparticles

Jung Sun Yoo, Hyeon Min Johng, Tae Jong Yoon, Hak Soo Shin, Byung Cheon Lee, Changhoon Lee, Byung Soo Ahn, Dae In Kang, Jin Kyu Lee, Kwang Sup Soh

Research output: Journal article publicationJournal articleAcademic researchpeer-review

25 Citations (Scopus)

Abstract

We describe the in vivo fluorescence imaging method of novel threadlike tissues (Bonghan ducts) inside the lymphatic vessels of rats with fluorescent magnetic nanoparticles. This threadlike structure was not visible by a stereomicroscope because of its transparency. Its thickness was about 20 μm and floated in the lymphatic fluid without adhering to the lymphatic wall. Injecting the nanoparticles into the lymph nodes and applying static magnetic fields on the lymphatic vessels, we were able to obtain the in vivo fluorescence imaging of the threadlike structures under the fluorescence reflectance imaging system. The specimen was analyzed with a confocal laser scanning microscopes and transmission electron microscopes which exhibited the preferential absorption of the nanoparticles by the threadlike structures compared with the lymphatic walls. This preferential absorption was due to the loose extracellular matrix of the threadlike structures. These results show new applications of nanoparticles for in vivo imaging of hardly detectable tissues using fluorescence reflectance imaging and magnetophoretic control.
Original languageEnglish
Pages (from-to)342-348
Number of pages7
JournalCurrent Applied Physics
Volume7
Issue number4
DOIs
Publication statusPublished - 1 May 2007
Externally publishedYes

Keywords

  • Acupuncture
  • Bonghan duct
  • Electron microscope
  • Lymphatic vessel
  • Nanoparticle

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy(all)

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