In vivo DTI assessment of hepatic ischemia reperfusion injury in an experimental rat model

Jerry S. Cheung, Juan Fan Shu, April M. Chow, Edward S. Hui, Ed X. Wu (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

31 Citations (Scopus)

Abstract

Purpose: To investigate hepatic ischemia reperfusion injury (IRI) using diffusion tensor imaging (DTI). Materials and Methods: Ten Sprague-Dawley rats were scanned at 7 Tesla (T) with DTI using b-value of 1000 s/mm2 and 6 gradient directions before, 2 h, and 1 day after 30-min total hepatic IRI. Apparent diffusion coefficient or mean diffusivity (MD), directional diffusivities and fractional anisotropy (FA) were measured. Seven of the animals were also examined with spin-echo echo-planar diffusion-weighted imaging (DWI) with seven b-values up to 2000 s/mm2 to estimate the true diffusion coefficient (D), blood pseudodiffusion coefficient (D*), and perfusion fraction (f) using a bi-compartmental model. Results: MD 2 h after IRI (0.77 ± 0.07 × 10-3 mm2/s) was significantly lower (P < 0.01) than that before (1.03 ± 0.07 × 10-3 mm2/s) and 1 day after IRI (1.01 ± 0.05 × 10 -3 mm2/s). Meanwhile, FA 2 h after IRI (0.33 ± 0.03) was significantly higher (P < 0.01) than that before (0.21 ± 0.02) and 1 day after IRI (0.20 ± 0.02). The bi-compartmental model analysis revealed the transient decrease in D, D* and f 2 h after IRI. Liver histology showed the multifocal cell swelling 3 h after IRI and widespread cell necrosis/apoptosis 1 day after IRI. Sinusoidal narrowing and congestion of erythrocytes were also observed 3 h and 1 day after IRI. Conclusion: DTI can characterize hepatic IRI by detecting the transient change in both MD and FA.

Original languageEnglish
Pages (from-to)890-895
Number of pages6
JournalJournal of Magnetic Resonance Imaging
Volume30
Issue number4
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

Keywords

  • Diffusion tensor imaging
  • Diffusion-weighted imaging
  • Fractional anisotropy
  • Ischemic reperfusion injury
  • IVIM
  • Liver
  • Mean diffusivity
  • MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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