In vitro anti-cancer activity of a novel microbial fermentation product on human carcinomas

Chung Hin Chui, Roberto Gambari, Fung Yi Lau, Gregory Yin Ming Cheng, Raymond Siu Ming Wong, Stanton Hon Lung Kok, Cheuk On Tang, Ivy Tuang Ngo Teo, Filly Cheung, Chor Hing Cheng, Kwok Ping Ho, Albert Sun Chi Chan, Alfonso Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)

Abstract

The possible anti-proliferation and cell death induction potential of a novel microbial fermentation extract named as oncogen XP-180 (or simply as XP-180) was tested on three human solid tumour carcinoma cell lines (non-small cell lung cancer A549, breast cancer MDA-MB231, liver adenocarcinoma SK-Hep1) and on the acute myelogenous leukaemia KG1a cell line. Anti-proliferative activity of XP-180 was observed on all of these cancer cell lines with comparable efficiency and in a dose-dependent manner. Morphological investigation further suggested that common features of apoptosis, including cell shrinkage and rounding, are present in XP-180 treated cells. Loss of adhesion properties of these solid tumour cell lines was observed upon XP-180 incubation. Anchorage-dependent clonogenicity assay on solid tumour cell lines and semi-solid methyl-cellulose colony formation assay on leukaemia cell line further revealed that XP-180 strongly inhibited the regeneration potential of these cancer cells. Using KG1a as an experimental model system, XP-180 was shown to stimulate the activity of caspase 3, 8 and 9 without significant change in caspase 6 activity. Furthermore, XP-180 readily induced collapse of mitochondrial membrane potential after 2 h of incubation. However, the use of the generic caspase specific inhibitor Z-VAD-FMK does not significantly reverse XP-180 mediated cell death. The results obtained suggest that XP-180-mediated cancer cell death could involve mitochondria and both caspase-dependent and -independent pathways. Therefore, XP-180 is an efficient anti-cancer regimen in vitro.
Original languageEnglish
Pages (from-to)675-679
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume17
Issue number4
Publication statusPublished - 1 Apr 2006

Keywords

  • Apoptosis
  • Neoplasms
  • Oncogen XP-180

ASJC Scopus subject areas

  • Genetics

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