In situ deposition of MOF-74(Cu) nanosheet arrays onto carbon cloth to fabricate a sensitive and selective electrocatalytic biosensor and its application for the determination of glucose in human serum

Shuisheng Hu, Yuxia Lin, Jing Teng, Wing Leung Wong (Corresponding Author), Bin Qiu

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7 Citations (Scopus)


A new electrocatalytic biosensor (MOF-74(Cu) NS-CC) based on the in situ deposition of MOF-74(Cu) nanosheet on carbon cloth via a bottom-up synthetic approach in a glass tube was developed. The electrocatalytic activity of the deposited MOF-74(Cu) NS was demonstrated in the oxidation of glucose to gluconate under alkaline conditions. The results revealed that the proposed method of in situ formation of MOF-74(Cu) NS onto a carbon cloth surface in a multi-layer solution is capable to generate a stable MOF-74(Cu) NS-CC electrode with excellent sensing performance. When the as-synthesized MOF-74(Cu) NS-CC was applied directly as the working electrode for glucose sensing, it showed much higher conductivity and redox activity than MOF-74(Cu) NS-GCE. With the potential applied at 0.55 V (vs. Ag/AgCl), this new electrocatalytic biosensor exhibits an excellent linear relationship between current density and concentration of glucose. Moreover, a wide linear range of detection (1.0 to 1000 μM) was observed. The limit of detection was found to be 0.41 μM (S/N = 3). The response sensitivity is 3.35 mA mM−1 cm−2 when the concentration of glucose is in the range 1–100 μM and 3.81 mA mM−1 cm−2 for the 100–1000 μM concentration range. This study provides a low-cost, easy to prepare, and reproducible methodology for the synthesis of highly redox-active nanomaterials based on the in situ formation of two-dimensional MOF-74(Cu) NS for the development of new electrocatalytic biosensors. [Figure not available: see fulltext.]

Original languageEnglish
Article number670
JournalMicrochimica Acta
Issue number12
Publication statusPublished - Dec 2020


  • Bottom-up synthesis
  • Carbon cloth
  • Electrochemical biosensor
  • Electrochemical catalysis
  • Glucose oxidation
  • Glucose sensor
  • In situ deposition
  • MOF-74(Cu)
  • Non-enzymatic method
  • Two-dimensional metal-organic framework nanosheet

ASJC Scopus subject areas

  • Analytical Chemistry

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