Impedance studies of bio-behavior and chemosensitivity of cancer cells by micro-electrode arrays

Qingjun Liu, Jinjiang Yu, Lidan Xiao, Cheuk On Tang, Yu Zhang, Ping Wang, Mo Yang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

105 Citations (Scopus)

Abstract

Impedimetric analysis on adherently growing cells by micro-electrodes provides information related to cell number, cell adhesion and cellular morphology. In this study, cell-based biosensor with micro-electrode arrays (MEAs) was used to monitor the culture behavior of mammalian cancer cells and evaluate the chemosensitivity of anti-cancer drugs using electrochemical impedance spectroscopy. The platinum electrode arrays were fabricated by semiconductor technology to a 10 × 10 pattern, with diameter of 80 μm of each electrode. The human oesophageal cancer cell lines (KYSE 30) were cultured on the surface of the electrodes with the pre-coated fibronectin, the connecting protein for tumor cells metastasis and adhesion in extracellular matrix. Morphology changes during cells adhesion, spreading, and proliferation can be detected by impedimetric analysis in a real time and non-invasive way. Cisplatin was added to cells for potential drug screening applications. The experimental results show that this well-known anti-cancer drug has characteristic chemosensitivity effects on KYSE 30 cells which can be detected by MEA. Thus, this cell-based chip provides a useful analytical method for cancer research.
Original languageEnglish
Pages (from-to)1305-1310
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number5
DOIs
Publication statusPublished - 1 Jan 2009

Keywords

  • Anti-cancer drug
  • Cancer
  • Cell-based biosensor
  • Chemosensitivity
  • Impedance spectroscopy
  • Micro-electrode arrays

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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