Immunostaining for Human Herpesvirus 8 Latent Nuclear Antigen-1 Helps Distinguish Kaposi Sarcoma from Its Mimickers

Wah Cheuk, Kathy O.Y. Wong, Sze Chuen Cesar Wong, J. E. Dinkel, David Ben-Dor, John K.C. Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

114 Citations (Scopus)

Abstract

We assessed the usefulness of a mouse monoclonal antibody (13B10) against human herpesvirus 8 (HHV-8) latent nuclear antigen-1 (LNA-1) in diagnosis of Kaposi sarcoma (KS) and for distinguishing it from various mimickers by studying 50 cases of KS and 53 mimickers (angiosarcoma, 15; kaposiform hemangioendothelioma, 6; spindle cell hemangioma, 3; reactive angioendotheliomatosis, 3; bacillary angiomatosis, 4; acroangiomatous dermatitis, 2; microvenular hemangioma, 2; hobnail hemangioma, 2; pyogenic granuloma, 5; dermatofibroma, 8; arteriovenous hemangioma, 1; verrucous hemangioma, 1; nonspecific vascular proliferation, 1) from patients with or without acquired HIV infection. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections. All 50 cases of KS were positive for HHV-8 LNA-1, with immunolocalization in the nuclei of the spindle cells and cells lining the primitive and thin-walled vascular channels, whereas all 53 mimickers (including 4 lesions from HIV-positive patients) tested negative. The results indicate that positive immunostaining for HHV-8 LNA-1 exhibits high sensitivity and specificity for the diagnosis of KS and is, thus, useful for distinguishing it from the mimickers.
Original languageEnglish
Pages (from-to)335-342
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume121
Issue number3
DOIs
Publication statusPublished - 1 Jan 2004
Externally publishedYes

Keywords

  • Angiosarcoma
  • Human herpesvirus 8
  • Immunohistochemistry
  • Kaposi sarcoma
  • Latent nuclear antigen-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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