TY - JOUR
T1 - Immunomodulation and antioxidant effects of anthocyanins from cherries on adjuvant-induced arthritis in rats
AU - He, Ying Hui
AU - Zhou, Jing
AU - Wang, Yue Sheng
AU - Xiao, Cheng
AU - Tong, Yan
AU - Tang, Cheuk On
AU - Chan, Albert Sun Chi
AU - Lu, Ai Ping
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Objective: The effects of anthocyanins from cherries on adjuvant-induced arthritis (AIA) in rats were investigated by measuring the levels of tumour necrosis factor-α (TNFα), interleukin-6 (IL-6), prostaglandin E2(PGE2) and by measuring the antioxidant effects. Materials and Methods: Arthritis was induced intradermally by injection with 0.1mL of complete Freund's adjuvant into the right hind footpad in male Sprague Dawley® (SD) rats. Anthocyanins at 300, 150 and 75 mg/kg bodyweight were administered orally to the treated rats once daily for 28 days after the injection. The swellings in the left hind paws were assayed for arthritic index. TNFα, IL-6 in serum and PGE2in paws were assayed by radioimmunoassay, and the antioxidant effects were assayed by testing superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. Results: Anthocyanins could significantly reduce the paw swelling from day 15 to day 28 after the injection, decrease the levels of TNFα, IL-6 and MDA in serum and PGE2in paws, and increase activity of SOD in serum on day 28 after the injection. Conclusions: The anthocyanins could protect against AIA in SD rats, and the mechanism works partly by regulating the inflammatory cytokines and improving the antioxidative status of AIA.
AB - Objective: The effects of anthocyanins from cherries on adjuvant-induced arthritis (AIA) in rats were investigated by measuring the levels of tumour necrosis factor-α (TNFα), interleukin-6 (IL-6), prostaglandin E2(PGE2) and by measuring the antioxidant effects. Materials and Methods: Arthritis was induced intradermally by injection with 0.1mL of complete Freund's adjuvant into the right hind footpad in male Sprague Dawley® (SD) rats. Anthocyanins at 300, 150 and 75 mg/kg bodyweight were administered orally to the treated rats once daily for 28 days after the injection. The swellings in the left hind paws were assayed for arthritic index. TNFα, IL-6 in serum and PGE2in paws were assayed by radioimmunoassay, and the antioxidant effects were assayed by testing superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. Results: Anthocyanins could significantly reduce the paw swelling from day 15 to day 28 after the injection, decrease the levels of TNFα, IL-6 and MDA in serum and PGE2in paws, and increase activity of SOD in serum on day 28 after the injection. Conclusions: The anthocyanins could protect against AIA in SD rats, and the mechanism works partly by regulating the inflammatory cytokines and improving the antioxidative status of AIA.
UR - http://www.scopus.com/inward/record.url?scp=31144457508&partnerID=8YFLogxK
U2 - 10.2165/01197065-200502020-00005
DO - 10.2165/01197065-200502020-00005
M3 - Journal article
SN - 1176-2330
VL - 2
SP - 95
EP - 99
JO - Evidence-Based Integrative Medicine
JF - Evidence-Based Integrative Medicine
IS - 2
ER -