Abstract
Delivery of cell-associated antigen represents an important strategy for vaccination. While many experimental models have been developed in order to define the critical parameters for efficient cross-priming, few have utilized quantitative methods that permit the study of the endogenous repertoire. Comparing different strategies of immunization, we report that local delivery of cell-associated antigen results in delayed T cell cross-priming due to the increased time required for antigen capture and presentation. In comparison, deliv-ery of disseminated antigen resulted in rapid T cell priming. Surprisingly, local injection of cell-associated antigen, while slower, resulted in the differentiation of a more robust, polyfunctional, effector response. We also evaluated the combination of cell-associated antigen with poly I:C delivery and observed an immunization route-specific effect regard-ing the optimal timing of innate immune stimulation.These studies highlight the importance of considering the timing and persistence of antigen presentation, and suggest that intra-dermal injection with delayed adjuvant delivery is the optimal strategy for achieving CD8+T cell cross-priming.
Original language | English |
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Article number | Article 71 |
Journal | Frontiers in Immunology |
Volume | 2 |
Issue number | DEC |
DOIs | |
Publication status | Published - 1 Dec 2011 |
Externally published | Yes |
Keywords
- Adjuvant delivery
- Cross-priming
- Dentritic cells
- Polyfunctional T cells
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology