Identification of the Transactivating Region of the Homeodomain Protein, Hex

Shinya Kasamatsu; Ayuko Sato; Taichi Yamamoto; Wee-Keong Vincent Keng; Hiroshi Yoshida; Yuka Yamazaki; Masafumi Shimoda; Jun Ichi Miyazaki; Tamio Noguchi

doi:10.1093/jb/mvh025

Identification of the Transactivating Region of the Homeodomain Protein, Hex

Shinya Kasamatsu, Ayuko Sato, Taichi Yamamoto, Wee-Keong Vincent Keng, Hiroshi Yoshida, Yuka Yamazaki, Masafumi Shimoda, Jun Ichi Miyazaki, Tamio Noguchi

Research output: Journal article publication › Journal article › Academic research › peer-review

16 Citations (Scopus)

Abstract

The homeodomain-containing protein Hex acts as an activator as well as a repressor of transcription in animals. While its repression domain has been mapped to the amino-terminal region, the activation domain has never been identified. Here, we show that the homeodomain and the acidic carboxyl-terminal region are necessary for full activation of the sodium-dependent bile acid cotransporter gene promoter in a cell type-independent manner, suggesting that the carboxyl-terminal region comprising residues 197 to 271 functions as the activation domain. In addition, we observed that a Hex mutant without this activation domain acts as a dominant-negative mutant as to the transactivating function of Hex.

Original language	English
Pages (from-to)	217-223
Number of pages	7
Journal	Journal of Biochemistry
Volume	135
Issue number	2
DOIs	https://doi.org/10.1093/jb/mvh025
Publication status	Published - 1 Feb 2004
Externally published	Yes

Keywords

Dominant-negative mutant
Hex
Sodium-dependent bile acid cotransporter
Transcriptional activator

ASJC Scopus subject areas

Biochemistry
Molecular Biology

More information

10.1093/jb/mvh025

Cite this

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AU - Kasamatsu, Shinya

AU - Sato, Ayuko

AU - Yamamoto, Taichi

AU - Keng, Wee-Keong Vincent

AU - Yoshida, Hiroshi

AU - Yamazaki, Yuka

AU - Shimoda, Masafumi

AU - Miyazaki, Jun Ichi

AU - Noguchi, Tamio

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AB - The homeodomain-containing protein Hex acts as an activator as well as a repressor of transcription in animals. While its repression domain has been mapped to the amino-terminal region, the activation domain has never been identified. Here, we show that the homeodomain and the acidic carboxyl-terminal region are necessary for full activation of the sodium-dependent bile acid cotransporter gene promoter in a cell type-independent manner, suggesting that the carboxyl-terminal region comprising residues 197 to 271 functions as the activation domain. In addition, we observed that a Hex mutant without this activation domain acts as a dominant-negative mutant as to the transactivating function of Hex.

KW - Dominant-negative mutant

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KW - Sodium-dependent bile acid cotransporter

KW - Transcriptional activator

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Identification of the Transactivating Region of the Homeodomain Protein, Hex

Abstract

Keywords

ASJC Scopus subject areas

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