Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Chi Ho Lee; John Hon Kei Lum; Belinda Pik Yuen Cheung; Man Sau Wong; Yoki Kwok Chu Butt; Ming F. Tam; Wing Y. Chan; Chit Chow; Pak Kwan Hui; Francis S L Kwok; Chun Lap Samuel Lo; D. M. Fan

doi:10.1002/pmic.200401159

Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Chi Ho Lee
, John Hon Kei Lum
, Belinda Pik Yuen Cheung
, Man Sau Wong
, Yoki Kwok Chu Butt
, Ming F. Tam
, Wing Y. Chan
, Chit Chow
, Pak Kwan Hui
, Francis S L Kwok
, Chun Lap Samuel Lo
, D. M. Fan

Research output: Journal article publication › Journal article › Academic research › peer-review

44 Citations (Scopus)

Abstract

MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.

Original language	English
Pages (from-to)	1160-1166
Number of pages	7
Journal	Proteomics
Volume	5
Issue number	4
DOIs	https://doi.org/10.1002/pmic.200401159
Publication status	Published - 1 Mar 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer biomarker
Gastrointestinal cancer
Human heterogeneous nuclear ribonucleoprotein A2/B1
MG7

ASJC Scopus subject areas

Molecular Biology
Genetics

More information

10.1002/pmic.200401159

Cite this

Lee, C. H., Lum, J. H. K., Cheung, B. P. Y., Wong, M. S., Butt, Y. K. C., Tam, M. F., Chan, W. Y., Chow, C., Hui, P. K., Kwok, F. S. L., Lo, C. L. S., & Fan, D. M. (2005). Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7. Proteomics, 5(4), 1160-1166. https://doi.org/10.1002/pmic.200401159

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title = "Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7",

abstract = "MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.",

keywords = "Cancer biomarker, Gastrointestinal cancer, Human heterogeneous nuclear ribonucleoprotein A2/B1, MG7",

author = "Lee, \{Chi Ho\} and Lum, \{John Hon Kei\} and Cheung, \{Belinda Pik Yuen\} and Wong, \{Man Sau\} and Butt, \{Yoki Kwok Chu\} and Tam, \{Ming F.\} and Chan, \{Wing Y.\} and Chit Chow and Hui, \{Pak Kwan\} and Kwok, \{Francis S L\} and Lo, \{Chun Lap Samuel\} and Fan, \{D. M.\}",

year = "2005",

month = mar,

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doi = "10.1002/pmic.200401159",

language = "English",

volume = "5",

pages = "1160--1166",

journal = "Proteomics",

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publisher = "Wiley-VCH Verlag",

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}

Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7. / Lee, Chi Ho; Lum, John Hon Kei; Cheung, Belinda Pik Yuen et al.
In: Proteomics, Vol. 5, No. 4, 01.03.2005, p. 1160-1166.

Research output: Journal article publication › Journal article › Academic research › peer-review

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T1 - Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

AU - Lee, Chi Ho

AU - Lum, John Hon Kei

AU - Cheung, Belinda Pik Yuen

AU - Wong, Man Sau

AU - Butt, Yoki Kwok Chu

AU - Tam, Ming F.

AU - Chan, Wing Y.

AU - Chow, Chit

AU - Hui, Pak Kwan

AU - Kwok, Francis S L

AU - Lo, Chun Lap Samuel

AU - Fan, D. M.

PY - 2005/3/1

Y1 - 2005/3/1

N2 - MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.

AB - MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.

KW - Cancer biomarker

KW - Gastrointestinal cancer

KW - Human heterogeneous nuclear ribonucleoprotein A2/B1

KW - MG7

UR - https://www.scopus.com/pages/publications/20144386703

U2 - 10.1002/pmic.200401159

DO - 10.1002/pmic.200401159

M3 - Journal article

C2 - 15759317

SN - 1615-9853

VL - 5

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JO - Proteomics

JF - Proteomics

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ER -

Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

More information

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