Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Chi Ho Lee; John Hon Kei Lum; Belinda Pik Yuen Cheung; Man Sau Wong; Yoki Kwok Chu Butt; Ming F. Tam; Wing Y. Chan; Chit Chow; Pak Kwan Hui; Francis S L Kwok; Chun Lap Samuel Lo; D. M. Fan

doi:10.1002/pmic.200401159

Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Chi Ho Lee, John Hon Kei Lum, Belinda Pik Yuen Cheung, Man Sau Wong, Yoki Kwok Chu Butt, Ming F. Tam, Wing Y. Chan, Chit Chow, Pak Kwan Hui, Francis S L Kwok, Chun Lap Samuel Lo, D. M. Fan

Research output: Journal article publication › Journal article › Academic research › peer-review

43 Citations (Scopus)

Abstract

MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.

Original language	English
Pages (from-to)	1160-1166
Number of pages	7
Journal	Proteomics
Volume	5
Issue number	4
DOIs	https://doi.org/10.1002/pmic.200401159
Publication status	Published - 1 Mar 2005

Keywords

Cancer biomarker
Gastrointestinal cancer
Human heterogeneous nuclear ribonucleoprotein A2/B1
MG7

ASJC Scopus subject areas

Molecular Biology
Genetics

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/pmic.200401159

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Lee, C. H., Lum, J. H. K., Cheung, B. P. Y., Wong, M. S., Butt, Y. K. C., Tam, M. F., Chan, W. Y., Chow, C., Hui, P. K., Kwok, F. S. L., Lo, C. L. S., & Fan, D. M. (2005). Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7. Proteomics, 5(4), 1160-1166. https://doi.org/10.1002/pmic.200401159

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abstract = "MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.",

keywords = "Cancer biomarker, Gastrointestinal cancer, Human heterogeneous nuclear ribonucleoprotein A2/B1, MG7",

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Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7. / Lee, Chi Ho; Lum, John Hon Kei; Cheung, Belinda Pik Yuen et al.
In: Proteomics, Vol. 5, No. 4, 01.03.2005, p. 1160-1166.

Research output: Journal article publication › Journal article › Academic research › peer-review

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AU - Butt, Yoki Kwok Chu

AU - Tam, Ming F.

AU - Chan, Wing Y.

AU - Chow, Chit

AU - Hui, Pak Kwan

AU - Kwok, Francis S L

AU - Lo, Chun Lap Samuel

AU - Fan, D. M.

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AB - MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.

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Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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