Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

Chi Ho Lee, John Hon Kei Lum, Belinda Pik Yuen Cheung, Man Sau Wong, Yoki Kwok Chu Butt, Ming F. Tam, Wing Y. Chan, Chit Chow, Pak Kwan Hui, Francis S L Kwok, Chun Lap Samuel Lo, D. M. Fan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

40 Citations (Scopus)

Abstract

MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue. KGaA.
Original languageEnglish
Pages (from-to)1160-1166
Number of pages7
JournalProteomics
Volume5
Issue number4
DOIs
Publication statusPublished - 1 Mar 2005

Keywords

  • Cancer biomarker
  • Gastrointestinal cancer
  • Human heterogeneous nuclear ribonucleoprotein A2/B1
  • MG7

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this