Identification of inhibitors of bacterial RNA polymerase

Xiao Yang, Cong Ma, Peter J. Lewis

Research output: Journal article publicationReview articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

Very few clinically available antibiotics target bacterial RNA polymerase (RNAP) suggesting it is an underutilized target. The advent of detailed structural information of RNAP holoenzyme (HE) has allowed the design and in silico screening of novel transcription inhibitors. Here, we describe our approach for the design and testing of small molecule transcription inhibitors that work by preventing the interaction between the essential transcription initiation factor σ and RNAP. With the appropriate structural information this approach can be easily modified to other essential protein-protein interactions.
Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalMethods
Volume86
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Keywords

  • Protein-protein interaction
  • RNA polymerase
  • Small molecule inhibitors
  • Transcription initiation

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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