Identification of a novel Wee1 isoform

Yaping Yu; Biao Chen; Zheng Chen; Cathy Fan; Yifan Han; Jing Zhang; Lizhi He; Alistair Ingram; Anil Kapoor; Jerry H. Wang; Damu Tang

doi:10.1016/j.bbaexp.2005.02.006

Identification of a novel Wee1 isoform

Yaping Yu, Biao Chen, Zheng Chen, Cathy Fan, Yifan Han, Jing Zhang, Lizhi He, Alistair Ingram, Anil Kapoor, Jerry H. Wang, Damu Tang

Research output: Journal article publication › Review article › Academic research › peer-review

2 Citations (Scopus)

Abstract

We have identified a novel isoform of Wee1 kinase (Wee1i), which uses Met215 of Wee1 as its initiation codon. RT-PCR, Western blot, and in situ hybridization verified wee1i expression in mammalian cells, rat brain, and rat thymus. Recombinant and partially purified Wee1i from rat thymus displayed kinase activity comparable to or higher than Wee1. The N-terminal 214 residues of Wee1 facilitate its ubiquitin-dependent degradation to trigger mitotic entry. Since Wee1i, lack of these 214 residues, it may evade this degradation and thus provide constitutive Wee1-like kinase activity to inhibit mitotic cell proliferation. Thus, Wee1i may play an important role in differentiation and in tumor suppression.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1729
Issue number	1
DOIs	https://doi.org/10.1016/j.bbaexp.2005.02.006
Publication status	Published - 25 May 2005
Externally published	Yes

Keywords

Cell cycle regulation
Cyclin-dependent kinase
Differentiation
Neuron
Wee1
Wee1 isoform

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

More information

10.1016/j.bbaexp.2005.02.006

Cite this

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title = "Identification of a novel Wee1 isoform",

abstract = "We have identified a novel isoform of Wee1 kinase (Wee1i), which uses Met215 of Wee1 as its initiation codon. RT-PCR, Western blot, and in situ hybridization verified wee1i expression in mammalian cells, rat brain, and rat thymus. Recombinant and partially purified Wee1i from rat thymus displayed kinase activity comparable to or higher than Wee1. The N-terminal 214 residues of Wee1 facilitate its ubiquitin-dependent degradation to trigger mitotic entry. Since Wee1i, lack of these 214 residues, it may evade this degradation and thus provide constitutive Wee1-like kinase activity to inhibit mitotic cell proliferation. Thus, Wee1i may play an important role in differentiation and in tumor suppression.",

keywords = "Cell cycle regulation, Cyclin-dependent kinase, Differentiation, Neuron, Wee1, Wee1 isoform",

author = "Yaping Yu and Biao Chen and Zheng Chen and Cathy Fan and Yifan Han and Jing Zhang and Lizhi He and Alistair Ingram and Anil Kapoor and Wang, {Jerry H.} and Damu Tang",

year = "2005",

month = may,

day = "25",

doi = "10.1016/j.bbaexp.2005.02.006",

language = "English",

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journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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T1 - Identification of a novel Wee1 isoform

AU - Yu, Yaping

AU - Chen, Biao

AU - Chen, Zheng

AU - Fan, Cathy

AU - Han, Yifan

AU - Zhang, Jing

AU - He, Lizhi

AU - Ingram, Alistair

AU - Kapoor, Anil

AU - Wang, Jerry H.

AU - Tang, Damu

PY - 2005/5/25

Y1 - 2005/5/25

N2 - We have identified a novel isoform of Wee1 kinase (Wee1i), which uses Met215 of Wee1 as its initiation codon. RT-PCR, Western blot, and in situ hybridization verified wee1i expression in mammalian cells, rat brain, and rat thymus. Recombinant and partially purified Wee1i from rat thymus displayed kinase activity comparable to or higher than Wee1. The N-terminal 214 residues of Wee1 facilitate its ubiquitin-dependent degradation to trigger mitotic entry. Since Wee1i, lack of these 214 residues, it may evade this degradation and thus provide constitutive Wee1-like kinase activity to inhibit mitotic cell proliferation. Thus, Wee1i may play an important role in differentiation and in tumor suppression.

AB - We have identified a novel isoform of Wee1 kinase (Wee1i), which uses Met215 of Wee1 as its initiation codon. RT-PCR, Western blot, and in situ hybridization verified wee1i expression in mammalian cells, rat brain, and rat thymus. Recombinant and partially purified Wee1i from rat thymus displayed kinase activity comparable to or higher than Wee1. The N-terminal 214 residues of Wee1 facilitate its ubiquitin-dependent degradation to trigger mitotic entry. Since Wee1i, lack of these 214 residues, it may evade this degradation and thus provide constitutive Wee1-like kinase activity to inhibit mitotic cell proliferation. Thus, Wee1i may play an important role in differentiation and in tumor suppression.

KW - Cell cycle regulation

KW - Cyclin-dependent kinase

KW - Differentiation

KW - Neuron

KW - Wee1

KW - Wee1 isoform

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U2 - 10.1016/j.bbaexp.2005.02.006

DO - 10.1016/j.bbaexp.2005.02.006

M3 - Review article

C2 - 15804487

SN - 0167-4781

VL - 1729

SP - 1

EP - 9

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Identification of a novel Wee1 isoform

Abstract

Keywords

ASJC Scopus subject areas

More information

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