Identification of a novel Wee1 isoform

Yaping Yu, Biao Chen, Zheng Chen, Cathy Fan, Yifan Han, Jing Zhang, Lizhi He, Alistair Ingram, Anil Kapoor, Jerry H. Wang, Damu Tang

Research output: Journal article publicationReview articleAcademic researchpeer-review

2 Citations (Scopus)

Abstract

We have identified a novel isoform of Wee1 kinase (Wee1i), which uses Met215 of Wee1 as its initiation codon. RT-PCR, Western blot, and in situ hybridization verified wee1i expression in mammalian cells, rat brain, and rat thymus. Recombinant and partially purified Wee1i from rat thymus displayed kinase activity comparable to or higher than Wee1. The N-terminal 214 residues of Wee1 facilitate its ubiquitin-dependent degradation to trigger mitotic entry. Since Wee1i, lack of these 214 residues, it may evade this degradation and thus provide constitutive Wee1-like kinase activity to inhibit mitotic cell proliferation. Thus, Wee1i may play an important role in differentiation and in tumor suppression.
Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1729
Issue number1
DOIs
Publication statusPublished - 25 May 2005
Externally publishedYes

Keywords

  • Cell cycle regulation
  • Cyclin-dependent kinase
  • Differentiation
  • Neuron
  • Wee1
  • Wee1 isoform

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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