Identification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Sze Chuen Cesar Wong, Vicky Wai-Ki Wong, Charles Ming-Lok Chan, Brigette Buig-Yue Ma, Edwin Pun Hui, Manson Chi-Keung Wong, Money Yan-Yee Lam, Thomas Chi-Chuen, Wing Han Chan, Wah Cheuk, Anthony Tak-Cheung Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

49 Citations (Scopus)

Abstract

Colorectal cancer (CRC) is the second most prevalent cause of cancer-related deaths in the Western world. 5-Fluorouracil (5-FU) is a standard chemotherapeutic drug to treat CRC. However, the response rate is less than 20% and patients who have responded to 5-FU may become resistant. Therefore there is an urgent need to examine the 5-FU response proteins so that patients with no response to 5-FU can change to other treatment strategies promptly. In this study, the proteomic expression profile in a CRC cell line SW480 before and after 5-FU treatment was examined using 2-dimensional electrophoresis technology. Fourteen proteins with differential expression were identified using mass spectrometry and 7 of them were validated using immunocytochemical (ICC) staining. Protein identification indicated that cyclophilin A, cytokeratin 19 (CK19), cytokeratin 8 (CK8), ras-related nuclear protein, heat shock protein 27 (hsp27) and peroxiredoxin 6 (Prx 6) were upregulated whereas heat shock protein 60 (hsp60), cytokeratin 18 (CK18), cytokeratin 9 (CK9), carbamoylphosphate synthetase I, α-enolase, heat shock protein 70 (hsp70), nm23 and β-actin were down-regulated. Seven of the 14 proteins detected were validated by ICC staining, which showed that the expression of hsp27, Prx 6 and hsp70 correlated with that from proteomics profiling. Our results suggest that hsp27, Prx 6 and hsp70 are potential 5-FU response proteins and they may represent potential targets for further evaluation in other 5-FU-sensitive and -resistant CRC cell lines.
Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalOncology Reports
Volume20
Issue number1
Publication statusPublished - 1 Jul 2008
Externally publishedYes

Keywords

  • 5-fluorouracil response proteins
  • Colorectal cancer
  • Mass spectrometry
  • SW480
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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