TY - JOUR
T1 - Hyperbranched Phosphorescent Conjugated Polymer Dots with Iridium(III) Complex as the Core for Hypoxia Imaging and Photodynamic Therapy
AU - Feng, Zhiying
AU - Tao, Peng
AU - Zou, Liang
AU - Gao, Pengli
AU - Liu, Yuan
AU - Liu, Xing
AU - Wang, Hua
AU - Liu, Shujuan
AU - Dong, Qingchen
AU - Li, Jie
AU - Xu, Bingshe
AU - Huang, Wei
AU - Wong, Wai Yeung
AU - Zhao, Qiang
PY - 2017/8/30
Y1 - 2017/8/30
N2 - Real-time monitoring of the contents of molecular oxygen (O2) in tumor cells is of great significance in early diagnosis of cancer. At the same time, the photodynamic therapy (PDT) could be realized by highly toxic singlet oxygen (1O2) generated in situ during the O2sensing, making it one of the most promising methods for cancer therapy. Herein, the iridium(III) complex cored hyperbranched phosphorescent conjugated polymer dots with the negative charges for hypoxia imaging and highly efficient PDT was rationally designed and synthesized. The incomplete energy transfer between the polyfluorene and the iridium(III) complexes realized the ratiometric sensing of O2for the accurate measurements. Furthermore, the O2-dependent emission lifetimes are also used in photoluminescence lifetime imaging and time-gated luminescence imaging for eliminating the autofluorescence remarkably to enhance the signal-to-noise ratio of imaging. Notably, the polymer dots designed could generate the1O2effectively in aqueous solution, and the image-guided PDT of the cancer cells was successfully realized and investigated in detail by confocal laser scanning microscope. To the best of our knowledge, this represents the first example of the iridium(III) complex cored hyperbranched conjugated polymer dots with the negative charges for both hypoxia imaging and PDT of cancer cells simultaneously.
AB - Real-time monitoring of the contents of molecular oxygen (O2) in tumor cells is of great significance in early diagnosis of cancer. At the same time, the photodynamic therapy (PDT) could be realized by highly toxic singlet oxygen (1O2) generated in situ during the O2sensing, making it one of the most promising methods for cancer therapy. Herein, the iridium(III) complex cored hyperbranched phosphorescent conjugated polymer dots with the negative charges for hypoxia imaging and highly efficient PDT was rationally designed and synthesized. The incomplete energy transfer between the polyfluorene and the iridium(III) complexes realized the ratiometric sensing of O2for the accurate measurements. Furthermore, the O2-dependent emission lifetimes are also used in photoluminescence lifetime imaging and time-gated luminescence imaging for eliminating the autofluorescence remarkably to enhance the signal-to-noise ratio of imaging. Notably, the polymer dots designed could generate the1O2effectively in aqueous solution, and the image-guided PDT of the cancer cells was successfully realized and investigated in detail by confocal laser scanning microscope. To the best of our knowledge, this represents the first example of the iridium(III) complex cored hyperbranched conjugated polymer dots with the negative charges for both hypoxia imaging and PDT of cancer cells simultaneously.
KW - hyperbranched polymer dots
KW - hypoxia imaging
KW - iridium(III) complexes
KW - phosphorescence
KW - photodynamic therapy
UR - http://www.scopus.com/inward/record.url?scp=85028667087&partnerID=8YFLogxK
U2 - 10.1021/acsami.7b09721
DO - 10.1021/acsami.7b09721
M3 - Journal article
SN - 1944-8244
VL - 9
SP - 28319
EP - 28330
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 34
ER -