TY - JOUR
T1 - Hydroxysafflor Yellow a Promotes Angiogenesis in Rat Brain Microvascu-lar Endothelial Cells Injured by Oxygen-glucose Deprivation/reoxygena-tion(OGD/R) through SIRT1-HIF-1α-VEGFA Signaling Pathway
AU - Ruan, Juxuan
AU - Wang, Lei
AU - Dai, Jiheng
AU - Li, Jing
AU - Wang, Ning
AU - Seto, Saiwang
N1 - Funding Information:
The study was supported by the National Natural Science Foundation of China (No.81773933), natural science research projects of Anhui Province higher learning in 2020 provided by Anhui Province Office of Education (KJ2020A0410). Anhui Province 13th batch 115 industrial innovation team “Innovative team for prevention and treatment of encephalopathy with acupuncture and medicine” project (Anhui talent Office [2020] No.4). Academic assistance program for the top-notch innovative talents from universities in 2017 provided by Anhui Province Office of Education (gxbjZD15).
Publisher Copyright:
© 2021 Bentham Science Publishers.
PY - 2021/12/8
Y1 - 2021/12/8
N2 - Objective: Angiogenesis led by brain microvascular endothelial cells (BMECs) con-tributes to the remission of brain injury after brain ischemia reperfusion. In this study, we investigated the effects of hydroxysafflor yellow A(HSYA) on angiogenesis of BMECs injured by OGD/R via SIRT1-HIF-1α-VEGFA signaling pathway. Methods: The OGD/R model of BMECs was established in vitro by OGD for 2h and reoxygena-tion for 24h. At first, the concentrations of vascular endothelial growth factor (VEGF), Angiopoi-etin (ang) and platelet-derived growth factor (PDGF) in supernatant were detected by ELISA, and the proteins expression of VEGFA, Ang-2 and PDGFB in BMECs were tested by western blot; the proliferation, adhesion, migration (scratch healing and transwell) and tube formation experiment of BMECs; the expression of CD31 and CD34 were tested by immunofluorescence staining. The levels of sirtuin1(SIRT1), hypoxia-inducible factor-1α (HIF-1α), VEGFA mRNA and protein were tested. Results: HSYA up-regulated the levels of VEGF, Ang and PDGF in the supernatant of BMECs un-der OGD/R, and the protein expression of VEGFA, Ang-2 and PDGFB was increased; HSYA could significantly alleviate the decrease of cell proliferation, adhesion, migration and tube formation ability of BMECs during OGD/R; HSYA enhanced the fluorescence intensity of CD31 and CD34 of BMECs during OGD/R; HSYA remarkably up-regulated the expression of SIRT1, HIF-1α, VEGFA mRNA and protein after OGD/R, and these increase decreased after SIRT1 was inhibited. Conclusion: SIRT1-HIF-1α-VEGFA signaling pathway is involved in HSYA improves angiogene-sis of BMECs injured by OGD/R.
AB - Objective: Angiogenesis led by brain microvascular endothelial cells (BMECs) con-tributes to the remission of brain injury after brain ischemia reperfusion. In this study, we investigated the effects of hydroxysafflor yellow A(HSYA) on angiogenesis of BMECs injured by OGD/R via SIRT1-HIF-1α-VEGFA signaling pathway. Methods: The OGD/R model of BMECs was established in vitro by OGD for 2h and reoxygena-tion for 24h. At first, the concentrations of vascular endothelial growth factor (VEGF), Angiopoi-etin (ang) and platelet-derived growth factor (PDGF) in supernatant were detected by ELISA, and the proteins expression of VEGFA, Ang-2 and PDGFB in BMECs were tested by western blot; the proliferation, adhesion, migration (scratch healing and transwell) and tube formation experiment of BMECs; the expression of CD31 and CD34 were tested by immunofluorescence staining. The levels of sirtuin1(SIRT1), hypoxia-inducible factor-1α (HIF-1α), VEGFA mRNA and protein were tested. Results: HSYA up-regulated the levels of VEGF, Ang and PDGF in the supernatant of BMECs un-der OGD/R, and the protein expression of VEGFA, Ang-2 and PDGFB was increased; HSYA could significantly alleviate the decrease of cell proliferation, adhesion, migration and tube formation ability of BMECs during OGD/R; HSYA enhanced the fluorescence intensity of CD31 and CD34 of BMECs during OGD/R; HSYA remarkably up-regulated the expression of SIRT1, HIF-1α, VEGFA mRNA and protein after OGD/R, and these increase decreased after SIRT1 was inhibited. Conclusion: SIRT1-HIF-1α-VEGFA signaling pathway is involved in HSYA improves angiogene-sis of BMECs injured by OGD/R.
KW - Angiogenesis
KW - BMECs
KW - HIF-1α
KW - HSYA
KW - OGD/R
KW - SIRT1
KW - VEGFA
UR - http://www.scopus.com/inward/record.url?scp=85123387627&partnerID=8YFLogxK
U2 - 10.2174/1567202618666211109104419
DO - 10.2174/1567202618666211109104419
M3 - Journal article
C2 - 34751117
AN - SCOPUS:85123387627
SN - 1567-2026
VL - 18
SP - 415
EP - 426
JO - Current Neurovascular Research
JF - Current Neurovascular Research
IS - 4
ER -