Homeobox gene Hex is essential for onset of mouse embryonic liver development and differentiation of the monocyte lineage

Wee-Keong Vincent Keng, Hideshi Yagi, Masahito Ikawa, Takashi Nagano, Zaw Myint, Kazuya Yamada, Takashi Tanaka, Ayuko Sato, Ikunobu Muramatsu, Masaru Okabe, Makoto Sato, Tamio Noguchi

Research output: Journal article publicationJournal articleAcademic researchpeer-review

139 Citations (Scopus)

Abstract

Disruption of the mouse Hex gene resulted in embryonic lethality around embryonic age (E) 10.5, due to no substantial liver formation. Expression of albumin Was detectable in heterozygous (Hex(±)) but not in homozygous (Hex(±)) embryos at E8.5. Instead of liver bud formation at E9.5, a liver-like capsule structure was observed in Hex(±) embryos. In Hex(±) mutant liver, we found no hepatocytes but no signs of apoptotic cell death in the area. Expression of transcription factors involved in hepatocyte differentiation, hepatocyte nuclear factor (Hnf)3β, Hnf6, Hnf4α and Hnf1α, were restricted to the capsule and internal matrix-like structure in the mutant liver and expression of a subset of these factors were reduced. Hematopoiesis of monocytes was impaired in mutant embryos while erythroid lineage was unaffected. These results indicate that Hex plays an essential role in progenitor cells which commit to the hepatic endoderm and in the hematopoietic differentiation of the monocyte lineage. (C) 2000 Academic Press.
Original languageEnglish
Pages (from-to)1155-1161
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number3
DOIs
Publication statusPublished - 5 Oct 2000
Externally publishedYes

Keywords

  • Gene targeting
  • Hematopoiesis
  • Hepatic endoderm differentiation
  • Hepatocyte differentiation
  • Hex

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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