Highly Sensitive and Selective Aptamer-Based Fluorescence Detection of a Malarial Biomarker Using Single-Layer MoS2 Nanosheets

Kenry, Alisha Geldert, Xiao Zhang, Hua Zhang, Chwee Teck Lim

Research output: Journal article publicationJournal articleAcademic researchpeer-review

47 Citations (Scopus)

Abstract

We demonstrate a highly selective and sensitive aptamer-based capture-release fluorescence detection of a malarial biomarker, i.e., Plasmodium lactose dehydrogenase (pLDH) protein, using single-layer two-dimensional MoS2 nanosheets. The detection of the target pLDH protein utilizing the aptamer-nanosheet sensing platform is rapid and can be easily completed within 10 min. In addition, the developed biomolecular sensor is capable of detecting the target malarial biomarker in homogeneous protein solutions as well as in heterogeneous mixtures of proteins. We anticipate that the ultrathin MoS2 nanosheet-based biosensor will facilitate the further development of biomolecular nanosensors for the detection of a wide range of biomarkers for malaria and other diseases.

Original languageEnglish
Pages (from-to)1315-1321
Number of pages7
JournalACS Sensors
Volume1
Issue number11
DOIs
Publication statusPublished - 23 Nov 2016
Externally publishedYes

Keywords

  • 2D nanomaterials
  • aptamers
  • malarial biomarker
  • MoS
  • Plasmodium lactate dehydrogenase (pLDH)
  • transition metal dichalcogenides

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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