Highly Sensitive and Selective Aptamer-Based Fluorescence Detection of a Malarial Biomarker Using Single-Layer MoS2 Nanosheets

Kenry; Alisha Geldert; Xiao Zhang; Hua Zhang; Chwee Teck Lim

doi:10.1021/acssensors.6b00449

Highly Sensitive and Selective Aptamer-Based Fluorescence Detection of a Malarial Biomarker Using Single-Layer MoS₂ Nanosheets

Kenry, Alisha Geldert, Xiao Zhang, Hua Zhang, Chwee Teck Lim

Research output: Journal article publication › Journal article › Academic research › peer-review

65 Citations (Scopus)

Abstract

We demonstrate a highly selective and sensitive aptamer-based capture-release fluorescence detection of a malarial biomarker, i.e., Plasmodium lactose dehydrogenase (pLDH) protein, using single-layer two-dimensional MoS₂ nanosheets. The detection of the target pLDH protein utilizing the aptamer-nanosheet sensing platform is rapid and can be easily completed within 10 min. In addition, the developed biomolecular sensor is capable of detecting the target malarial biomarker in homogeneous protein solutions as well as in heterogeneous mixtures of proteins. We anticipate that the ultrathin MoS₂ nanosheet-based biosensor will facilitate the further development of biomolecular nanosensors for the detection of a wide range of biomarkers for malaria and other diseases.

Original language	English
Pages (from-to)	1315-1321
Number of pages	7
Journal	ACS Sensors
Volume	1
Issue number	11
DOIs	https://doi.org/10.1021/acssensors.6b00449
Publication status	Published - 23 Nov 2016
Externally published	Yes

Keywords

2D nanomaterials
aptamers
malarial biomarker
MoS
Plasmodium lactate dehydrogenase (pLDH)
transition metal dichalcogenides

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1021/acssensors.6b00449

Cite this

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title = "Highly Sensitive and Selective Aptamer-Based Fluorescence Detection of a Malarial Biomarker Using Single-Layer MoS2 Nanosheets",

abstract = "We demonstrate a highly selective and sensitive aptamer-based capture-release fluorescence detection of a malarial biomarker, i.e., Plasmodium lactose dehydrogenase (pLDH) protein, using single-layer two-dimensional MoS2 nanosheets. The detection of the target pLDH protein utilizing the aptamer-nanosheet sensing platform is rapid and can be easily completed within 10 min. In addition, the developed biomolecular sensor is capable of detecting the target malarial biomarker in homogeneous protein solutions as well as in heterogeneous mixtures of proteins. We anticipate that the ultrathin MoS2 nanosheet-based biosensor will facilitate the further development of biomolecular nanosensors for the detection of a wide range of biomarkers for malaria and other diseases.",

keywords = "2D nanomaterials, aptamers, malarial biomarker, MoS, Plasmodium lactate dehydrogenase (pLDH), transition metal dichalcogenides",

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AU - Zhang, Hua

AU - Lim, Chwee Teck

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